Integrated lipidomic and transcriptomic analysis reveals phospholipid changes in somatic embryos of Picea asperata in response to partial desiccation
Date
2022
Authors
Ling, Juanjuan
Xia, Yan
Hu, Jiwen
Zhu, Tianqing
Wang, Junhui
Zhang, Hanguo
Kong, Lisheng
Journal Title
Journal ISSN
Volume Title
Publisher
International Journal of Molecular Sciences
Abstract
Partial desiccation treatment (PDT) is an effective technology for promoting the germination
and conversion of conifer somatic embryos (SEs). PDT, as a drought stress, induces intensive
physiological responses in phospholipid metabolism, which are not well understood in the conifer SEs.
Here, we integrated lipidomics, transcriptomics and proteomics analyses to reveal the molecular basis
of lipid remodeling under PDT in Picea asperata SEs. Among the 82 lipid molecular species determined
by mass spectrometry, phosphatidic acid (PA) had a significant effect after PDT and was the most
critical lipid in the response to PDT. The transcriptomics results showed that multiple transcripts in
the glycerolipid and glycerophospholipid metabolism pathways were differentially expressed, and
these included five PLDα1 transcripts that catalyze the conversion of phosphatidylcholine (PC) to
PA. Furthermore, the enzyme activity of this phospholipase D (PLD) was significantly enhanced in
response to PDT, and PDT also significantly increased the protein level of PLDα1 (MA_10436582g0020).
In addition, PA is a key factor in gibberellin, abscisic acid and ethylene signal transduction. One
GDI1, one DELLA, three ABI1s, two SnRK2s, one CTR and 12 ERFs showed significantly differential
expression between SEs before and after PDT in this study. Our data suggest that the observed
increases in the PA contents might result from the activation of PLDα by PDT. PA not only affects the
physical and chemical properties of the cell membrane but also participates in plant hormone signal
transduction. Our work provides novel insight into the molecular mechanism through which PDT
promotes the germination of SEs of coniferous tree species and fills the gap in the understanding of
the mechanism of somatic embryo lipid remodeling in response to PDT.
Description
Keywords
Picea asperata, somatic embryo, partial desiccation treatment, phospholipids, phospholipid acid, Centre for Forest Biology
Citation
Ling, J., Xia, Y., Hu, J., Zhu, T., Wang, J., . . . Kong, L. (2022). “Integrated lipidomic and transcriptomic analysis reveals phospholipid changes in somatic embryos of Picea asperata in response to partial desiccation.” International Journal of Molecular Sciences, 23(12), 6494. https://doi.org/10.3390/ijms23126494