Poplar MYB115 and MYB134 Transcription Factors Regulate Proanthocyanidin Synthesis and Structure

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dc.contributor.authorJames, Amy Midori
dc.contributor.authorMa, Dawei
dc.contributor.authorMellway, Robin
dc.contributor.authorGesell, Andreas
dc.contributor.authorYoshida, Kazuko
dc.contributor.authorWalker, Vincent
dc.contributor.authorTran, Lan
dc.contributor.authorStewart, Don
dc.contributor.authorReichelt, Michael
dc.contributor.authorSuvanto, Jussi
dc.contributor.authorSalminen, Juha-Pekka
dc.contributor.authorGershenzon, Jonathan
dc.contributor.authorSéguin, Armand
dc.contributor.authorConstabel, C. Peter
dc.date.accessioned2018-08-15T16:25:59Z
dc.date.available2018-08-15T16:25:59Z
dc.date.copyright2017en_US
dc.date.issued2017
dc.description.abstractThe accumulation of proanthocyanidins is regulated by a complex of transcription factors composed of R2R3 MYB, basic helix-loop-helix, and WD40 proteins that activate the promoters of biosynthetic genes. In poplar (genus Populus), MYB134 is known to regulate proanthocyanidin biosynthesis by activating key flavonoid genes. Here, we characterize a second MYB regulator of proanthocyanidins, MYB115. Transgenic poplar overexpressing MYB115 showed a high-proanthocyanidin phenotype and reduced salicinoid accumulation, similar to the effects of MYB134 overexpression. Transcriptomic analysis of MYB115-and MYB134-overexpressing poplar plants identified a set of common up-regulated genes encoding proanthocyanidin biosynthetic enzymes and several novel uncharacterized MYB transcriptional repressors. Transient expression experiments demonstrated the capacity of both MYB134 and MYB115 to activate flavonoid promoters, but only in the presence of a basic helix-loop-helix cofactor. Yeast two-hybrid experiments confirmed the direct interaction of these transcription factors. The unexpected identification of dihydromyricetin in leaf extracts of both MYB115-and MYB134-overexpressing poplar led to the discovery of enhanced flavonoid B-ring hydroxylation and an increased proportion of prodelphinidins in proanthocyanidin of the transgenics. The dramatic hydroxylation phenotype of MYB115 overexpressors is likely due to the up-regulation of both flavonoid 39,59-hydroxylases and cytochrome b(5). Overall, this work provides new insight into the complexity of the gene regulatory network for proanthocyanidin synthesis in poplar.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by the Natural Sciences and Engineering Research Council of Canada, the Max Planck Society, the Academy of Finland, and the Canadian Genomics R&D Initiative.en_US
dc.identifier.citationJames, A.M.; Ma, D.; Mellway, R.; Gesell, A.; Yoshida, K.; Walker, V.; … & Constabel, C.P. (2017). Poplar MYB115 and MYB134 transcription factors regulate proanthocyanidin synthesis and structure. Plant Physiology, 174(1), 154-171. https://doi.org/10.1104/pp.16.01962en_US
dc.identifier.urihttps://doi.org/10.1104/pp.16.01962
dc.identifier.urihttp://hdl.handle.net/1828/9905
dc.language.isoenen_US
dc.publisherPlant Physiologyen_US
dc.subjectCentre for Forest Biology
dc.subject.departmentDepartment of Biology
dc.titlePoplar MYB115 and MYB134 Transcription Factors Regulate Proanthocyanidin Synthesis and Structureen_US
dc.typeArticleen_US

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