Controlled release of glial cell line-derived neurotrophic factor from poly (ε-caprolactone) microspheres

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dc.contributor.authorAgbay, Andrew
dc.contributor.authorMohtaram, Nima Khadem
dc.contributor.authorWillerth, Stephanie
dc.date.accessioned2016-05-24T12:19:59Z
dc.date.available2016-05-24T12:19:59Z
dc.date.copyright2014en_US
dc.date.issued2014
dc.description.abstractGlial cell line-derived neurotrophic factor (GDNF), a growth factor expressed in the central nervous system, promotes the survival of both dopaminergic and motor neurons, making it a promising candidate for neurodegenerative disease therapy. Although GDNF is currently being evaluated in clinical trials for the treatment of Parkinson’s disease (PD), the current delivery method using catheter implantation has certain limitations in terms of delivering GDNF safely and effectively. As a proof of concept, we encapsulated GDNF into poly(ε-caprolactone) (PCL) microspheres to enable controlled drug release for 25 days. First, microspheres were loaded with bovine serum albumin (BSA) to determine the optimal fabrication conditions necessary to achieve the desired release rates of protein. BSA was then used as a carrier protein to preserve GDNF activity during the fabrication process in the presence of organic solvents. GDNF-encapsulated microspheres were created and characterized using scanning electron microscopy. Next, the in vitro release of GDNF along with microsphere morphology was tracked over 25 days. Finally, the bioactivity of the released GDNF was confirmed using PC12 cells. This work demonstrates the potential of such microspheres for the delivery of bioactive GDNF with the end goal of developing a suitable, clinically relevant formulation for injection to appropriate regions of the brain in PD patients.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe authors would like to acknowledge support from an NSERC Discovery Grant (S.M.W.) and an NSERC Engage Grant with MedGenesis Therapeutix. They would also like to acknowledge the Advanced Microscopy Facility at the University of Victoria and MedGenesis Therapeutix for their on-going support of this project.en_US
dc.identifier.citationAgbay, A., Mohtaram, N.K. & Willerth, S.M. (2014). Controlled release of glial cell line-derived neurotrophic factor from poly (ε-caprolactone) microspheres. Drug Delivery and Translational Research, 4(2), 159-170.en_US
dc.identifier.urihttp://dx.doi.org/10.1007/s13346-013-0189-0
dc.identifier.urihttp://hdl.handle.net/1828/7310
dc.language.isoenen_US
dc.publisherDrug Delivery and Translational Researchen_US
dc.subjectGlial Cell Line-Derived Neurotrophic Factor (GDNF)en_US
dc.subjectParkinson’s disease (PD)en_US
dc.subjectMicrospheresen_US
dc.subjectControlled Releaseen_US
dc.subjectSpinal cord injuryen_US
dc.titleControlled release of glial cell line-derived neurotrophic factor from poly (ε-caprolactone) microspheresen_US
dc.typePostprinten_US

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