Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar

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dc.contributor.authorYasuike, Motoshige
dc.contributor.authorDe Boer, Johan
dc.contributor.authorVon Schalburg, Kristian Robert
dc.contributor.authorCooper, Glenn A.
dc.contributor.authorMcKinnel, Linda
dc.contributor.authorMessmer, Amber
dc.contributor.authorSo, Stacy
dc.contributor.authorDavidson, William S.
dc.contributor.authorKoop, Benjamin F.
dc.date.accessioned2013-11-29T23:32:12Z
dc.date.available2013-11-29T23:32:12Z
dc.date.copyright2010en_US
dc.date.issued2010-09-02
dc.descriptionBioMed Centralen_US
dc.description.abstractBackground: The Atlantic salmon (Salmo salar) immunoglobulin heavy chain (IgH) locus possesses two parallel IgH isoloci (IGH-A and IGH-B), that are related to the genomic duplication event in the family Salmonidae. These duplicated IgH loci in Atlantic salmon provide a unique opportunity to examine the mechanisms of genome diversity and genome evolution of the IgH loci in vertebrates. In this study, we defined the structure of these loci in Atlantic salmon, and sequenced 24 bacterial artificial chromosome (BAC) clones that were assembled into the IGH-A (1.1 Mb) and IGH-B (0.9 Mb) loci. In addition, over 7,000 cDNA clones from the IgH variable (VH) region have been sequenced and analyzed. Results: The present study shows that the genomic organization of the duplicated IgH loci in Atlantic salmon differs from that in other teleosts and other vertebrates. The loci possess multiple Cτ genes upstream of the Cμ region, with three of the Cτ genes being functional. Moreover, the duplicated loci possess over 300 VH segments which could be classified into 18 families. This is the largest number of VH families currently defined in any vertebrate. There were significant structural differences between the two loci, indicating that both IGH-A and -B loci have evolved independently in the short time after the recent genome duplication approximately 60 mya. Conclusions: Our results indicate that the duplication of the IgH loci in Atlantic salmon significantly contributes to the increased diversity of the antibody repertoire, as compared with the single IgH locus in other vertebrates.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by Genome Canada, Genome BC and by the Natural Sciences and Engineering Research Council of Canada (B.K., W.D).en_US
dc.identifier.citationYasuike et al.: Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar. BMC Genomics 2010 11:486.en_US
dc.identifier.urihttp://www.biomedcentral.com/1471-2164/11/486
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2164-11-486
dc.identifier.urihttp://hdl.handle.net/1828/5057
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.subject.departmentDepartment of Biology
dc.titleEvolution of duplicated IgH loci in Atlantic salmon, Salmo salaren_US
dc.typeArticleen_US

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