Molecular determinants of cysteine string protein modulation of N-type calcium channels




Miller, Linda C.
Swayne, Leigh Anne
Kay, Jason G.
Feng, Zhong-Ping
Jarvis, Scott E.
Zamponi, Gerald W.
Braun, Janice E. A.

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Journal of Cell Science


Cysteine string proteins (CSPs) are secretory vesicle chaperones that are important for neurotransmitter release. We have previously reported an interaction of CSP with both heterotrimeric GTP-binding proteins (G proteins) and N-type calcium channels that results in a tonic G protein inhibition of the channels. In this report we directly demonstrate that two separate regions of CSP associate with G proteins. The N-terminal binding site of CSP, which includes the J domain, binds Ga subunits but not Gbg subunits whereas the C terminal binding site of CSP associates with either free Gbg subunits or Gbg in complex with Ga. The interaction of either binding site of CSP (CSP1-82 or CSP83-198) with G proteins elicits robust tonic inhibition of N-type calcium channel activity. However, CSP1-82 inhibition and CSP83-198 inhibition of calcium channels occur through distinct mechanisms. Calcium channel inhibition by CSP83-198 (but not CSP1-82) is completely blocked by co-expression of the synaptic protein interaction site (synprint) of the N-type channel, indicating that CSP83-198 inhibition is dependent on a physical interaction with the calcium channel. These results suggest that distinct binding sites of CSP can play a role in modulating G protein function and G protein inhibition of calcium channels.



Cysteine string protein, Chaperones, G proteins, N-type calcium channels, Synaptic transmission, J domain


Miller, L.C., Swayne, L.A., Kay, J.G., Feng, Z-P., Jarvis, S.E., Zamponi, G.W. & Braun, J.E.A. (2003). Molecular determinants of cysteine string protein modulation of N-type calcium channels. Journal of Cell Science, 116(14), 2967-2974.