Utilizing gold nanoparticles as prospective radiosensitizers in 3D radioresistant pancreatic co-culture model

Simple item page
dc.contributor.authorAlhussan, Abdulaziz
dc.contributor.authorJackson, Nolan
dc.contributor.authorCalisin, Reinali
dc.contributor.authorMorgan, Jessica
dc.contributor.authorBeckham, Wayne
dc.contributor.authorChithrani, Devika B.
dc.date.accessioned2024-01-24T21:36:04Z
dc.date.available2024-01-24T21:36:04Z
dc.date.copyright2023en_US
dc.date.issued2023
dc.description.abstractPancreatic cancer stands among the deadliest forms of cancer, and the existing treatments fall short of providing adequate efficacy. Novel and more effective treatment approaches are urgently required to address this critical medical challenge. In this study, we aimed to evaluate the anti-cancer efficacy of gold nanoparticles (GNPs) in combination with radiotherapy (RT). A 3D pancreatic cancer co-culture spheroid model of MIA PaCa-2 cancer cells and patient-derived cancer-associated fibroblasts (CAF-98) was used. The spheroids were treated with GNPs (7.5 μg/mL) and 2 Gy of RT. The spheroids’ cell viability was assessed through the CellTiter-Glo 3D assay, and an immunofluorescence assay was used to assess the DNA DSBs via the expression of the DNA damage marker 53BP1. Co-culture samples showed a 10.8% (p < 0.05) increase in proliferation and a 13.0% (p < 0.05) decrease in DNA DSB when compared to monoculture samples, However, they displayed a 175% (p < 0.001) increase in GNPs uptake when compared to monoculture spheroids. Using GNPs/RT, we were able to show a significant reduction of 6.2% (p < 0.05) in spheroid size and an increase of 14.3% (p < 0.05) in DNA DSB damage in co-culture samples. The combination of GNPs with RT demonstrated remarkable radiosensitization effects, representing a promising approach to enhance cancer treatment efficacy. These effects were particularly noteworthy in the more treatment-resistant co-culture spheroid model.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by the Kuwait Foundation for the Advancement of Sciences (KFAS) under project code CB21-63SP-01, NanoMedicines Innovation Network Strategic Initiative fund (NMIN-SI) a member of the Networks of Centres of Excellence Canada program, the John R. Evans Leaders Fund (JELF) from the Canada Foundation for Innovation (CFI), British Columbia Knowledge Development Fund (BCKDF), the Discovery grant from the Natural Sciences and Engineering Research Council of Canada (NSERC), and a collaborative health grant from the University of Victoria, and BC Cancer Victoria.en_US
dc.identifier.citationAlhussan, A., Jackson, N., Calisin, R., Morgan, J., Beckham, W., & Chithrani, D. B. (2023). Utilizing gold nanoparticles as prospective radiosensitizers in 3D Radioresistant pancreatic co-culture model. International Journal of Molecular Sciences, 24(15), 12523. https://doi.org/10.3390/ijms241512523en_US
dc.identifier.urihttps://doi.org/10.3390/ijms241512523
dc.identifier.urihttp://hdl.handle.net/1828/15866
dc.language.isoenen_US
dc.publisherInternational Journal of Molecular Sciencesen_US
dc.subjectgold nanoparticles
dc.subjectradiosensitizers
dc.subject3D spheroids
dc.subjectco-culture
dc.subjectpancreatic cancer
dc.subjectCentre for Advanced Materials and Related Technology (CAMTEC)
dc.subject.departmentDepartment of Physics and Astronomy
dc.subject.departmentDepartment of Biochemistry and Microbiology
dc.subject.departmentDepartment of Chemistry
dc.subject.departmentDivision of Medical Sciences
dc.subject.departmentSchool of Medical Sciences
dc.titleUtilizing gold nanoparticles as prospective radiosensitizers in 3D radioresistant pancreatic co-culture modelen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
alhussan_abdulaziz_int j mol sci_2023.pdf
Size:
6.5 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Faculty and Staff Publications
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.