Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts
| dc.contributor.author | Larina, Irina M. | |
| dc.contributor.author | Percy, Andrew J. | |
| dc.contributor.author | Yang, Juncong | |
| dc.contributor.author | Borchers, Christoph H. | |
| dc.contributor.author | Nosovsky, Andrei M. | |
| dc.contributor.author | Grigoriev, Anatoli I. | |
| dc.contributor.author | Nikolae, Evgeny N. | |
| dc.date.accessioned | 2018-11-20T17:25:35Z | |
| dc.date.available | 2018-11-20T17:25:35Z | |
| dc.date.copyright | 2017 | en_US |
| dc.date.issued | 2017 | |
| dc.description.abstract | The effects of spaceflight on human physiology is an increasingly studied field, yet the molecular mechanisms driving physiological changes remain unknown. With that in mind, this study was performed to obtain a deeper understanding of changes to the human proteome during space travel, by quantitating a panel of 125 proteins in the blood plasma of 18 Russian cosmonauts who had conducted long-duration missions to the International Space Station. The panel of labeled prototypic tryptic peptides from these proteins covered a concentration range of more than 5 orders of magnitude in human plasma. Quantitation was achieved by a well-established and highly-regarded targeted mass spectrometry approach involving multiple reaction monitoring in conjunction with stable isotope-labeled standards. Linear discriminant function analysis of the quantitative results revealed three distinct groups of proteins: 1) proteins with post-flight protein concentrations remaining stable, 2) proteins whose concentrations recovered slowly, or 3) proteins whose concentrations recovered rapidly to their pre-flight levels. Using a systems biology approach, nearly all of the reacting proteins could be linked to pathways that regulate the activities of proteases, natural immunity, lipid metabolism, coagulation cascades, or extracellular matrix metabolism. | en_US |
| dc.description.reviewstatus | Reviewed | en_US |
| dc.description.scholarlevel | Faculty | en_US |
| dc.description.sponsorship | This study was partly supported by the Russian Scientific Foundation (grant no. 14-24-00114). C.H.B., J.Y., and A.J.P. would like to thank Genome Canada and Genome BC for providing support to the University of Victoria-Genome BC Proteomics Centre through the Genome Innovations Network (204PRO for operations; 214PRO for technology development). C.H.B. is also grateful for support from the Leading Edge Endowment Fund and the Segal McGill Chair in Molecular Oncology at McGill University (Montreal, Quebec, Canada). C.H.B. is also grateful for support from the Warren Y. Soper Charitable Trust and the Alvin Segal Family Foundation to the Jewish General Hospital (Montreal, Quebec, Canada). | en_US |
| dc.identifier.citation | Larina, I.M.; Percy, A.J.; Yang, J.; Borchers, C.H.; Nosovsky, A.M.; Grigoriev, A.I.; & Nikolaev, E.N. (2017). Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts. Scientific Reports, 7, article 8142. DOI: 10.1038/s41598-017-08432-w | en_US |
| dc.identifier.uri | https://doi.org/10.1038/s41598-017-08432-w | |
| dc.identifier.uri | http://hdl.handle.net/1828/10335 | |
| dc.language.iso | en | en_US |
| dc.publisher | Scientific Reports | en_US |
| dc.subject | UVic Genome BC Proteomics Centre | |
| dc.subject.department | Department of Biochemistry and Microbiology | |
| dc.title | Protein expression changes caused by spaceflight as measured for 18 Russian cosmonauts | en_US |
| dc.type | Article | en_US |