Microglia modulate hippocampal synaptic transmission and sleep duration along the light/dark cycle

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dc.contributor.authorCorsi, Giorgio
dc.contributor.authorPicard, Katherine
dc.contributor.authordi Castro, Maria Amalia
dc.contributor.authorGarofalo, Stefano
dc.contributor.authorTucci, Federico
dc.contributor.authorChece, Giuseppina
dc.contributor.authordel Percio, Claudio
dc.contributor.authorGolia, Maria Teresa
dc.contributor.authorRaspa, Marcello
dc.contributor.authorScavizzi, Ferdinando
dc.contributor.authorDecoeur, Fanny
dc.contributor.authorLauro, Clotilde
dc.contributor.authorRigamonti, Mara
dc.contributor.authorIannello, Fabio
dc.contributor.authorRagozzino, Davide Antonio
dc.contributor.authorRusso, Eleonora
dc.contributor.authorBernardini, Giovanni
dc.contributor.authorNadjar, Agnès
dc.contributor.authorTremblay, Marie-Ève
dc.contributor.authorBabiloni, Claudio
dc.date.accessioned2022-04-01T17:04:08Z
dc.date.available2022-04-01T17:04:08Z
dc.date.copyright2022en_US
dc.date.issued2022
dc.description.abstractMicroglia, the brain's resident macrophages, actively contribute to the homeostasis of cerebral parenchyma by sensing neuronal activity and supporting synaptic remodeling and plasticity. While several studies demonstrated different roles for astrocytes in sleep, the contribution of microglia in the regulation of sleep/wake cycle and in the modulation of synaptic activity in the different day phases has not been deeply investigated. Using light as a zeitgeber cue, we studied the effects of microglial depletion with the colony stimulating factor-1 receptor antagonist PLX5622 on the sleep/wake cycle and on hippocampal synaptic transmission in male mice. Our data demonstrate that almost complete microglial depletion increases the duration of NREM sleep and reduces the hippocampal excitatory neurotransmission. The fractalkine receptor CX3CR1 plays a relevant role in these effects, because cx3cr1GFP/GFP mice recapitulate what found in PLX5622-treated mice. Furthermore, during the light phase, microglia express lower levels of cx3cr1 and a reduction of cx3cr1 expression is also observed when cultured microglial cells are stimulated by ATP, a purinergic molecule released during sleep. Our findings suggest that microglia participate in the regulation of sleep, adapting their cx3cr1 expression in response to the light/dark phase, and modulating synaptic activity in a phase-dependent manner.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipAgenzia di Ricerca per la Sclerosi Laterale Amiotrofica. Grant Number: NKINALS 2019 Associazione Italiana per la Ricerca sul Cancro. Grant Numbers: AIRC 22329, IG23010 Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada. Grant Number: RGPIN-05308-2014 Ministero dell'Istruzione, dell'Università e della Ricerca. Grant Number: PRIN2017/20178L7WRS University Bordeaux. Grant Number: ANR-10-LABX-43 Université de Bordeaux Natural Sciences and Engineering Research Council of Canada Ministero della Salute. Grant Number: RIcerca Finalizzata/20178L7WRSen_US
dc.identifier.citationCorsi, G., Picard, K., di Castro, M. A., Garofalo, S., Tucci, F., Chece, G., del Percio, C., Golia, M. T., Raspa, M., Scavizzi, F., Decoeur, F., Lauro, C., Rigamonti, M., Iannello, F., Ragozzino, D. A., Russo, E., Bernardini, G., Nadjar, A., Tremblay, M. E., … Limatola, C. (2022). Microglia modulate hippocampal synaptic transmission and sleep duration along the light/dark cycle. Glia, 70(1), 89– 105. https://doi.org/10.1002/glia.24090en_US
dc.identifier.urihttps://doi.org/10.1002/glia.24090
dc.identifier.urihttp://hdl.handle.net/1828/13838
dc.language.isoenen_US
dc.publisherGliaen_US
dc.subjectcx3cr1
dc.subjectelectroencephalography
dc.subjectlong-term potentiation
dc.subjectmicroglial depletion
dc.subjectminiature excitatory post-synaptic currents
dc.subjectsleep
dc.subjectspontaneous excitatory post-synaptic currents
dc.subject.departmentDivision of Medical Sciences
dc.subject.departmentSchool of Medical Sciences
dc.titleMicroglia modulate hippocampal synaptic transmission and sleep duration along the light/dark cycleen_US
dc.typeArticleen_US

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