Solvent Isotope Effect on Biomolecular Adsorption at Hydrophobic Surfaces

dc.contributor.authorJarisz, Tasha A.
dc.contributor.authorJena, Kailash C.
dc.contributor.authorDixon, Matthew C.
dc.contributor.authorHore, Dennis K.
dc.date.accessioned2020-01-04T18:35:08Z
dc.date.available2020-01-04T18:35:08Z
dc.date.copyright2017en_US
dc.date.issued2017
dc.description.abstractIn vibrational studies of biological systems, D2O is often substituted for H2O to more clearly resolve the amide I and C–H stretching regions. However, in interfacial studies where adsorption is involved, the difference between these two solvents may play a role in the amount adsorbed and, consequently, the adsorbed structure. Nonlinear vibrational spectroscopy reveals striking differences in the spectral signature of the hydrophobic amino acid leucine adsorbed on polystyrene in the presence of light versus heavy water. Dissipation-monitoring quartz crystal microbalance studies conclude that more amino acid is present on the hydrophobic surface when the solvent is D2O, and the relative surface populations account for the difference in spectral intensity. This finding has consequences for surface structural studies, and provides insight into the role of solvent in biomolecular adsorption at hydrophobic surfaces.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis project was supported by a Discovery Grant from the Natural Science and Engineering Research Council of Canada (NSERC). The SFG spectrometer was purchased with assistance from the Canadian Foundation for Innovation Leader’s Opportunity Fund, and the British Columbia Knowldege Development Fund. T.A.J. is grateful to NSERC for a PGS-M scholarship.en_US
dc.identifier.citationJarisz, T. A.; Jena, K. C.; Dison, M. C.; & Hore, D. K. (2017). Solvent isotope effect on biomolecular adsorption at hydrophobic surfaces. The Journal of Physical Chemistry C, 121(31), 16879-16887. DOI: 10.1021/acs.jpcc.7b05377en_US
dc.identifier.urihttps://doi.org/10.1021/acs.jpcc.7b05377
dc.identifier.urihttp://hdl.handle.net/1828/11453
dc.language.isoenen_US
dc.publisherThe Journal of Physical Chemistry Cen_US
dc.subjectInterfaces
dc.subjectAdsorption
dc.subjectNonlinear optics
dc.subjectHydrophobicity solvents
dc.subject.departmentDepartment of Chemistry
dc.titleSolvent Isotope Effect on Biomolecular Adsorption at Hydrophobic Surfacesen_US
dc.typePostprinten_US

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