Complex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination drops

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dc.contributor.authorPrior, Natalie
dc.contributor.authorLittle, Stefan
dc.contributor.authorBoyes, Ian
dc.contributor.authorGriffith, Patrick
dc.contributor.authorHusby, Chad
dc.contributor.authorPirone‑Davies, Cary
dc.contributor.authorStevenson, Dennis W.
dc.contributor.authorTomlinson, P. Barry
dc.contributor.authorvon Aderkas, Patrick
dc.date.accessioned2020-11-02T23:00:15Z
dc.date.available2020-11-02T23:00:15Z
dc.date.copyright2019en_US
dc.date.issued2019
dc.description.abstractPollination drops are aqueous secretions that receive pollen and transport it to the ovule interior in gymnosperms (Coniferales, Cycadales, Ginkgoales, Gnetales). Proteins are well established as components of pollination drops in conifers (Coniferales) and Ephedra spp. (Gnetales), but it is unknown whether proteins are also present in the pollination drops of cycads (Cycadales), Ginkgo (Ginkgoales), Gnetum (Gnetales), or in the pollination drops produced by sterile ovules occurring on pollen plants in the Gnetales. We used liquid chromatography–tandem mass spectrometry followed by database-derived protein identification to conduct proteomic surveys of pollination drops collected from: Ceratozamia hildae, Zamia furfuracea and Cycas rumphii (Cycadales); Ginkgo biloba (Ginkgoales); Gnetum gnemon and Welwitschia mirabilis, including pollination drops from both microsporangiate and ovulate plants (Gnetales). We identified proteins in all samples: C. hildae (61), Z. furfuracea (40), C. rumphii (9), G. biloba (57), G. gnemon ovulate (17) and sterile ovules from microsporangiate plants (25) and W. mirabilis fertile ovules (1) and sterile ovules from microsporangiate plants (138). Proteins involved in defence and carbohydrate modification occurred in the drops of most groups, indicating conserved functions for proteins in pollination drops. Our study demonstrates that all extant gymnosperm groups produce complex reproductive secretions containing proteins, an ancient trait that likely contributed to the evolutionary success of seed plants.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe authors would like to thank Julia Gill (University of Victoria), William Tang (United States Department of Agriculture), Michael Calonje (Montgomery Botanical Center), Derek Smith, Darryl Hardie, Monica Elliott, Jun Han, Jason Serpa (University of Victoria Genome British Columbia Proteomics Centre), Gane Ka-shu Wong of the 1 KP project for sharing its resources, and Kelly Sendall (Royal British Columbia Museum), as well as Sean Graham (University of British Columbia) for sharing their expert knowledge. Financial support was provided by the Natural Sciences and Engineering Research Council of Canada by way of a Post-Graduate Scholarship to NA Prior and a Discovery Program Grant (#43775-2013) to P. von Aderkas and National Science Foundation Grant IOS-0922738 to D. Stevenson. PvA acknowledges support as a Visiting Foreign Researcher of the University of the Ryukyus, Japan. The University of Victoria Graduate Centre for Forest Biology also provided travel support to NAP.en_US
dc.identifier.citationPrior, N., Little, S. A., Boyes, I., Griffith, P., Husby, C., Pirone‑Davies, C., … von Aderkas, P. (2019) Complex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination drops. Plant Reproduction, 2019(32). https://doi.org/10.1007/s00497-018-0348-zen_US
dc.identifier.urihttps://doi.org/10.1007/s00497-018-0348-z
dc.identifier.urihttp://hdl.handle.net/1828/12295
dc.language.isoenen_US
dc.publisherPlant Reproductionen_US
dc.subjectGymnosperms
dc.subjectPollination drops
dc.subjectCycads
dc.subjectGinkgo
dc.subjectGnetales
dc.subjectSpermatophytes
dc.subjectCentre for Forest Biology
dc.subject.departmentDepartment of Biology
dc.titleComplex reproductive secretions occur in all extant gymnosperm lineages: a proteomic survey of gymnosperm pollination dropsen_US
dc.typeArticleen_US

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