Two novel mitoviruses from a Canadian isolate of the Dutch elm pathogen Ophiostoma novo-ulmi (93–1224)

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dc.contributor.authorHintz, William E.
dc.contributor.authorCarneiro, Joyce S
dc.contributor.authorKassatenko, Irina
dc.contributor.authorVarga, Aniko
dc.contributor.authorJames, Delano
dc.date.accessioned2015-03-13T23:17:01Z
dc.date.available2015-03-13T23:17:01Z
dc.date.copyright2013en_US
dc.date.issued2013-08-08
dc.descriptionBioMed Centralen_US
dc.description.abstractBackground: Ophiostoma novo-ulmi is the causative agent of Dutch elm disease (DED). It is an ascomycetous filamentous fungus that ranks as the third most devastating fungal pathogen in Canada. The disease front has spread eastward and westward from the epicentre in Ontario and Quebec and is threatening elm populations across the country. Numerous mitigation strategies have been tried to eradicate this pathogen, but success has thus far been limited. An alternative approach might utilize double-stranded RNA (dsRNA) mycoviruses which have been reported to induce hypovirulence in other fungi. Methods: Using a modified single primer amplification technique (SPAT) in combination with chromosomal walking, we have determined the genome sequence of two RdRp encoding dsRNA viruses from an O. novo-ulmi isolate (93–1224) collected from the disease front in Winnipeg. Results: We propose that these viruses, which we have named OnuMV1c and OnuMV7 based on sequence similarity to other Ophiostoma mitoviruses, are two new members of the genus Mitovirus in the family Narnaviridae. Conclusions: The discovery of such dsRNA elements raises the potential for engineering these viruses to include other genetic elements, such as anti-sense or interfering RNAs, to create novel and highly specific biological controls. Naïve fungal hosts could be infected with both the engineered molecule and a helper mitovirus encoding an RdRp which would provide replication capacity for both molecules.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis research was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC) through the Discovery Grants Program.en_US
dc.identifier.citationHintz et al.: Two novel mitoviruses from a Canadian isolate of the Dutch elm pathogen Ophiostoma novo-ulmi (93–1224). Virology Journal 2013 10:252.en_US
dc.identifier.urihttp://www.virologyj.com/content/10/1/252
dc.identifier.urihttp://dx.doi.org/10.1186/1743-422X-10-252
dc.identifier.urihttp://hdl.handle.net/1828/5913
dc.language.isoenen_US
dc.publisherVirology Journalen_US
dc.rights.tempAttribution-NonCommercial-NoDerivs 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ca/*
dc.subjectOphiostoma novo-ulmi
dc.subjectdsRNA virus
dc.subjectHypovirulence
dc.subjectMitovirus
dc.subjectDutch elm disease
dc.subjectBiological control
dc.subjectCentre for Forest Biology
dc.subject.departmentDepartment of Biology
dc.titleTwo novel mitoviruses from a Canadian isolate of the Dutch elm pathogen Ophiostoma novo-ulmi (93–1224)en_US
dc.typeArticleen_US

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