The molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins

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dc.contributor.authorPercy, Andrew J.
dc.contributor.authorKashirina, Daria N.
dc.contributor.authorPastushkova, Liudmila Kh.
dc.contributor.authorBorchers, Christoph H.
dc.contributor.authorKireev, Kirill S.
dc.contributor.authorIvanisenko, Vladimir A.
dc.contributor.authorKononikhin, Alexey S.
dc.contributor.authorNikolaev, Eugene N.
dc.contributor.authorIrina M., Larina
dc.date.accessioned2020-10-28T21:56:17Z
dc.date.available2020-10-28T21:56:17Z
dc.date.copyright2019en_US
dc.date.issued2020-10-28
dc.description.abstractBackground The conditions of space flight have a significant effect on the physiological processes in the human body, yet the molecular mechanisms driving physiological changes remain unknown. Methods Blood samples of 18 Russian cosmonauts who had conducted long-duration missions to the International Space Station were collected 30 days before launch and on the first and seventh days after landing. Results A panel of 125 proteins in the blood plasma was quantitated by a well-established and highly regarded targeted mass spectrometry approach. This method involves the monitoring of multiple reactions in conjunction with stable isotope-labeled standards at the University of Victoria - Genome BC Proteomics Centre. Conclusions Reduction of circulating plasma volume during space flight and activation of fluid retention at the final stage of the flight affect the changes in plasma protein concentrations present in the first days after landing. Using an ANOVA approach, it was revealed that only 1 protein (S100A9) reliably responded to space flight conditions. This protein plays an important role in the functioning of the endothelium and can serve as a marker for activation of inflammatory reactions. Concentrations of the proteins of complement, coagulation cascades, and acute phase reactants increase in the blood of cosmonauts as measured the first day after landing. Most of these proteins’ concentrations continue to increase by the 7th day after space flight. Similar dynamics are observed for proteases and their inhibitors. Thus, there is a shift in proteolytic blood systems, which is necessary for the restoration of muscle tissue and maintenance of oncotic homeostasis.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipAcknowledgments The authors are grateful for Conference Organizing Committee BGRS\SB- 2018, ICG SB RAS (budget project 0324-2018-0017). We thank Gene L. Dowell, MD, MS, University of Texas Medical Branch and the reviewers for science discussion and valuable comments. Funding The research and publication costs were covered by RFBR grant № 18–34- 00524 (KDN) and Russian Ministry of Science and Education (project 28.12487.2018/12.1).en_US
dc.identifier.urihttps://doi.org/10.1186/s12920-019-0490-y
dc.identifier.urihttp://hdl.handle.net/1828/12277
dc.language.isoenen_US
dc.subjectCosmonauts
dc.subjectMass spectrometry
dc.subjectBlood proteins
dc.subjectUVic Genome BC Proteomics Centre
dc.titleThe molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteinsen_US
dc.typeArticleen_US

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