Investigation of Nano-Bio Interactions within a Pancreatic Tumor Microenvironment for the Advancement of Nanomedicine in Cancer Treatment
Date
2021
Authors
Alhussan, Abdulaziz
Bromma, Kyle
Bozdoğan, Ece Pinar Demirci
Metcalfe, Andrew
Karaskinska, Joanna
Beckham, Wayne
Alexander, Abraham S.
Renouf, Daniel J.
Schaeffer, David F.
Chithrani, Devika B.
Journal Title
Journal ISSN
Volume Title
Publisher
Current Oncolocy
Abstract
Pancreatic cancer is one of the deadliest types of cancer, with a five-year survival rate of
only 10%. Nanotechnology offers a novel perspective to treat such deadly cancers through their
incorporation into radiotherapy and chemotherapy. However, the interaction of nanoparticles (NPs)
with cancer cells and with other major cell types within the pancreatic tumor microenvironment
(TME) is yet to be understood. Therefore, our goal is to shed light on the dynamics of NPs within a
TME of pancreatic origin. In addition to cancer cells, normal fibroblasts (NFs) and cancer-associated
fibroblasts (CAFs) were examined in this study due to their important yet opposite roles of suppressing
tumor growth and promoting tumor growth, respectively. Gold nanoparticles were used
as the model NP system due to their biocompatibility and physical and chemical proprieties, and
their dynamics were studied both quantitatively and qualitatively in vitro and in vivo. The in vitro
studies revealed that both cancer cells and CAFs take up 50% more NPs compared to NFs. Most
importantly, they all managed to retain 70–80% of NPs over a 24-h time period. Uptake and retention
of NPs within an in vivo environment was also consistent with in vitro results. This study shows the
paradigm-changing potential of NPs to combat the disease.
Description
Keywords
pancreatic cancer, gold nanoparticles, uptake, retention, PANC-1, Mia PaCa-2, normal, Centre for Advanced Materials and Related Technologies (CAMTEC), Centre for Biomedical Research
Citation
Alhussan, A., Bromma, K., Bozdoğan, E. P. D., Metcalfe, A., Karasinska, J., Beckham, W., … Chithrani, D. B. (2021). Investigation of Nano-Bio Interactions within a Pancreatic Tumor Microenvironment for the Advancement of Nanomedicine in Cancer Treatment. Current Oncolocy, 28(3), 1-18. https://doi.org/10.3390/curroncol28030183.