Optimizing Differentiation Protocols for Producing Dopaminergic Neurons from Human Induced Pluripotent Stem Cells for Tissue Engineering Applications

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dc.contributor.authorRobinson, Meghan
dc.contributor.authorYau, Suk-yu
dc.contributor.authorSun, Lin
dc.contributor.authorGabers, Nicole
dc.contributor.authorBibault, Emma
dc.contributor.authorChristie, Brian R.
dc.contributor.authorWillerth, Stephanie
dc.date.accessioned2015-06-11T19:04:11Z
dc.date.available2015-06-11T19:04:11Z
dc.date.copyright2015en_US
dc.date.issued2015-03-05
dc.description.abstractParkinson’s disease (PD) is a neurodegenerative disorder that results when the dopaminergic neurons (DNs) present in the substantia nigra necessary for voluntary motor control are depleted, making patients with this disorder ideal candidates for cell replacement therapy. Human induced pluri-potent stem cells (hiPSCs), obtained by reprogramming adult cells, possess the properties of pluripotency and immortality while enabling the possibility of patient-specific therapies. An effective cell therapy for PD requires an efficient, defined method of DN generation, as well as protection from the neuroin-flammatory environment upon engraftment. Although similar in pluripotency to human embryonic stem cells (hESCs), hiPSCs differentiate less efficiently into neuronal subtypes. Previous work has shown that treatment with guggulsterone can efficiently differentiate hESCs into DNs. Our work shows that guggulsterone is able to derive DNs from hiPSCs with comparable efficiency, and furthermore, this differentiation can be achieved inside three-dimensional fibrin scaffolds that could enhance cell survival upon engraftment.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe authors acknowledge funding from the Natural Sciences and Engineering Research of Canada. MRMR was funded with support from a University of Victoria donor named Ann Gibson. SY is supported by a postdoctoral fellowship funded by the Canadian Institutes of Health Research in partnership with Fragile X Research Foundation of Canada.en_US
dc.identifier.citationRobinson et al. Optimizing Differentiation Protocols for Producing Dopaminergic Neurons from Human Induced Pluripotent Stem Cells for Tissue Engineering Applications. Biomarker Insights 2015:10(S1) 61–70en_US
dc.identifier.urihttp://dx.doi.org/10.4137/BMIMI.S20064
dc.identifier.urihttp://www.la-press.com/optimizing-differentiation-protocols-for-producing-dopaminergic-neuron-article-a4864
dc.identifier.urihttp://hdl.handle.net/1828/6256
dc.language.isoenen_US
dc.publisherLibertas Academia Ltden_US
dc.subjecttissue engineering
dc.subjectbiomaterials
dc.subjectpluripotent stem cells
dc.subjectdifferentiation
dc.subjectneuroscience
dc.subject.departmentDepartment of Mechanical Engineering
dc.subject.departmentSchool of Medical Sciences
dc.subject.departmentDepartment of Biology
dc.titleOptimizing Differentiation Protocols for Producing Dopaminergic Neurons from Human Induced Pluripotent Stem Cells for Tissue Engineering Applicationsen_US
dc.typeArticleen_US

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