The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration
| dc.contributor.author | da Silva, Victor A. | |
| dc.contributor.author | Bobotis, Bianca C. | |
| dc.contributor.author | Correia, Felipe F. | |
| dc.contributor.author | Lima-Vasconcellos, Théo H. | |
| dc.contributor.author | Chiarantin, Gabrielly M.D. | |
| dc.contributor.author | De La Vega, Laura | |
| dc.contributor.author | Lombello, Christane B. | |
| dc.contributor.author | Willerth, Stephanie M. | |
| dc.contributor.author | Malmonge, Sônia M. | |
| dc.contributor.author | Pachson, V. | |
| dc.date.accessioned | 2023-10-20T18:15:59Z | |
| dc.date.available | 2023-10-20T18:15:59Z | |
| dc.date.copyright | 2023 | en_US |
| dc.date.issued | 2023 | |
| dc.description.abstract | Tissue engineering for spinal cord injury (SCI) remains a complex and challenging task. Biomaterial scaffolds have been suggested as a potential solution for supporting cell survival and differentiation at the injury site. However, different biomaterials display multiple properties that significantly impact neural tissue at a cellular level. Here, we evaluated the behavior of different cell lines seeded on chitosan (CHI), poly ("-caprolactone) (PCL), and poly (L-lactic acid) (PLLA) scaffolds. We demonstrated that the surface properties of a material play a crucial role in cell morphology and differentiation. While the direct contact of a polymer with the cells did not cause cytotoxicity or inhibit the spread of neural progenitor cells derived from neurospheres (NPCdn), neonatal rat spinal cord cells (SCC) and NPCdn only attached and matured on PCL and PLLA surfaces. Scanning electron microscopy and computational analysis suggested that cells attached to the material’s surface emerged into distinct morphological populations. Flow cytometry revealed a higher differentiation of neural progenitor cells derived from human induced pluripotent stem cells (hiPSC-NPC) into glial cells on all biomaterials. Immunofluorescence assays demonstrated that PCL and PLLA guided neuronal differentiation and network development in SCC. Our data emphasize the importance of selecting appropriate biomaterials for tissue engineering in SCI treatment. | en_US |
| dc.description.reviewstatus | Reviewed | en_US |
| dc.description.scholarlevel | Faculty | en_US |
| dc.description.sponsorship | This research was supported by FAPESP (2019/17892-8 and 2020/11667-0), CNPq (312047/2017-7 and 315372/2021-4), CAPES, and UFABC. The following authors were recipients of the FAPESP research scholarships: V.A.d.S. (2018/19300-8), B.C.B. (2019/24406-2), F.F.C. (2018/06316-3, 2019/18921-1, 2018/06316-3, 2017/12112-9), and T.H.L.M. (2020/02035-0 and 2021/11969-9). G.M.D.C. was a recipient of a CNPq research fellowship (153268/2018-2). | en_US |
| dc.identifier.citation | Da Silva, V. A., Bobotis, B. C., Correia, F. F., De Lima-Vasconcellos, T. H., Chiarantin, G. M. D., De La Vega, L., Lombello, C. B., Willerth, S. M., Malmonge, S. M., Paschon, V., & Kihara, A. H. (2023a). The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration. International Journal of Molecular Sciences, 24(17), 13642. https://doi.org/10.3390/ijms241713642 | en_US |
| dc.identifier.uri | https://doi.org/10.3390/ijms241713642 | |
| dc.identifier.uri | http://hdl.handle.net/1828/15541 | |
| dc.language.iso | en | en_US |
| dc.publisher | International Journal of Molecular Science | en_US |
| dc.subject | biomaterial surface | |
| dc.subject | tissue therapy | |
| dc.subject | spinal cord injury | |
| dc.subject | cell replacement | |
| dc.subject | human induced pluripotent stem cells | |
| dc.subject | hiPSCs | |
| dc.subject.department | Department of Mechanical Engineering | |
| dc.subject.department | School of Medical Sciences | |
| dc.title | The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration | en_US |
| dc.type | Article | en_US |