Antimicrobial photodynamic inactivation of planktonic and biofilm cells by covalently immobilized porphyrin on polyethylene terephthalate surface

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dc.contributor.authorShatila, Fatima
dc.contributor.authorTieman, Grace M. O.
dc.contributor.authorMusolino, Stefania F.
dc.contributor.authorWulff, Jeremy E.
dc.contributor.authorBuckley, Heather L.
dc.date.accessioned2024-01-24T23:42:06Z
dc.date.available2024-01-24T23:42:06Z
dc.date.copyright2022en_US
dc.date.issued2022
dc.descriptionThe authors would like to thank the Department of Microbiology and Biochemistry for providing the bacterial strains, and the CAMTEC Advanced Microscopic Facility at the University of Victoria for providing training on the Scanning Electron Microscope and Confocal Laser Scanning Microscope. We also thank Rebecca Hof for support and access to CAMTEC BioCore facilities, Luiz Henrique da Silva Correa for his support with Imaris software, and Epic Ventures as partners in this research.en_US
dc.description.abstractThe appearance of resistant strains and the persistence of biofilms on different surfaces in a wide range of settings represent serious public health threats. Antimicrobial photodynamic inactivation (aPDI) is a promising alternative technology to overcome these challenges. The current study assessed the antimicrobial effect of polyethylene terephthalate (PET) discs covalently functionalized with a cationic porphyrin, against E. coli and P. aeruginosa growth. Irradiation with white LED light for 6 h resulted in 1.51 ± 0.03 and 3.26 ± 0.24 log reduction of planktonic P. aeruginosa and E. coli, respectively. The study also assessed the effect of the functionalized discs on biofilm formation by E. coli, P. aeruginosa, and S. aureus. The biovolumes of S. aureus, P. aeruginosa, and E. coli biofilms were decreased by 0.6 ± 0.1, 0.56 ± 0.13 and 0.74 ± 0.06 log reduction, respectively. These results emphasize the ability of porphyrin-functionalized photoactive surfaces to kill bacterial cells and consequently prevent biofilm formation.en_US
dc.description.reviewstatusUnrevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipWe gratefully acknowledge Mitacs Canada (grant IT17318) and our industry partners, Epic Ventures, as well as the IESVic Clean Energy Systems Accelerator Program (IESVic-CESAP) IRAP, for funding.en_US
dc.identifier.citationShatila, F., Tieman, G. M. O., Musolino, S. F., Wulff, J. E., & Buckley, H. L. (2022). Antimicrobial photodynamic inactivation of planktonic and biofilm cells by covalently immobilized porphyrin on polyethylene terephthalate surface. SSRN. https://dx.doi.org/10.2139/ssrn.4286188en_US
dc.identifier.urihttps://dx.doi.org/10.2139/ssrn.4286188
dc.identifier.urihttps://ssrn.com/abstract=4286188
dc.identifier.urihttp://hdl.handle.net/1828/15875
dc.language.isoenen_US
dc.publisherSSRNen_US
dc.subjectBiofilmsen_US
dc.subjectphotodynamic inactivationen_US
dc.subjectporphyrinen_US
dc.subjectphotoactive surfacesen_US
dc.subjectantimicrobial surfacesen_US
dc.titleAntimicrobial photodynamic inactivation of planktonic and biofilm cells by covalently immobilized porphyrin on polyethylene terephthalate surfaceen_US
dc.typePreprinten_US

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