Antimicrobial photodynamic inactivation of planktonic and biofilm cells by covalently immobilized porphyrin on polyethylene terephthalate surface
Date
2022
Authors
Shatila, Fatima
Tieman, Grace M. O.
Musolino, Stefania F.
Wulff, Jeremy E.
Buckley, Heather L.
Journal Title
Journal ISSN
Volume Title
Publisher
SSRN
Abstract
The appearance of resistant strains and the persistence of biofilms on different surfaces in a wide range of settings represent serious public health threats. Antimicrobial photodynamic inactivation (aPDI) is a promising alternative technology to overcome these challenges. The current study assessed the antimicrobial effect of polyethylene terephthalate (PET) discs covalently functionalized with a cationic porphyrin, against E. coli and P. aeruginosa growth. Irradiation with white LED light for 6 h resulted in 1.51 ± 0.03 and 3.26 ± 0.24 log reduction of planktonic P. aeruginosa and E. coli, respectively. The study also assessed the effect of the functionalized discs on biofilm formation by E. coli, P. aeruginosa, and S. aureus. The biovolumes of S. aureus, P. aeruginosa, and E. coli biofilms were decreased by 0.6 ± 0.1, 0.56 ± 0.13 and 0.74 ± 0.06 log reduction, respectively. These results emphasize the ability of porphyrin-functionalized photoactive surfaces to kill bacterial cells and consequently prevent biofilm formation.
Description
The authors would like to thank the Department of Microbiology and Biochemistry for providing the bacterial strains, and the CAMTEC Advanced Microscopic Facility at the University of Victoria for providing training on the Scanning Electron Microscope and Confocal Laser Scanning Microscope. We also thank Rebecca Hof for support and access to CAMTEC BioCore facilities, Luiz Henrique da Silva Correa for his support with Imaris software, and Epic Ventures as partners in this research.
Keywords
Biofilms, photodynamic inactivation, porphyrin, photoactive surfaces, antimicrobial surfaces
Citation
Shatila, F., Tieman, G. M. O., Musolino, S. F., Wulff, J. E., & Buckley, H. L. (2022). Antimicrobial photodynamic inactivation of planktonic and biofilm cells by covalently immobilized porphyrin on polyethylene terephthalate surface. SSRN. https://dx.doi.org/10.2139/ssrn.4286188