A 3D analysis revealed complexe mitochondria morphologies in porcine cumulus cells

Simple item page
dc.contributor.authorLounas, Amel
dc.contributor.authorLebrun, Ariane
dc.contributor.authorLaflamme, Isabelle
dc.contributor.authorVernoux, Nathalie
dc.contributor.authorSavage, Julie
dc.contributor.authorTremblay, Marie-Ève
dc.contributor.authorGermain, Marc
dc.contributor.authorRichard, François J.
dc.date.accessioned2023-01-13T23:31:53Z
dc.date.available2023-01-13T23:31:53Z
dc.date.copyright2022en_US
dc.date.issued2022
dc.description.abstractIn the ovarian follicle, a bilateral cell-to-cell communication exists between the female germ cell and the cumulus cells which surround the oocyte. This communication allows the transit of small size molecules known to impact oocyte developmental competence. Pyruvate derivatives produced by mitochondria, are one of these transferred molecules. Interestingly, mitochondria may adopt a variety of morphologies to regulate their functions. In this study, we described mitochondrial morphologies in porcine cumulus cells. Active mitochondria were stained with TMRM (Tetramethylrhodamine, Methyl Ester, Perchlorate) and observed with 2D confocal microscopy showing mitochondria of different morphologies such as short, intermediate, long, and very long. The number of mitochondria of each phenotype was quantified in cells and the results showed that most cells contained elongated mitochondria. Scanning electron microscopy (SEM) analysis confirmed at nanoscale resolution the different mitochondrial morphologies including round, short, intermediate, and long. Interestingly, 3D visualisation by focused ion-beam scanning electron microscopy (FIB-SEM) revealed different complex mitochondrial morphologies including connected clusters of different sizes, branched mitochondria, as well as individual mitochondria. Since mitochondrial dynamics is a key regulator of function, the description of the mitochondrial network organisation will allow to further study mitochondrial dynamics in cumulus cells in response to various conditions such as in vitro maturation.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.identifier.citationLounas, A., Lebrun, A., Laflamme, I., Vernoux, N., Savage, J., Tremblay, M., . . . Richard, F. (2022). “A 3D analysis revealed complexe mitochondria morphologies in porcine cumulus cells.” Scientific Reports, 12(15403). https://doi.org/10.1038/s41598-022-19723-2en_US
dc.identifier.urihttps://doi.org/10.1038/s41598-022-19723-2
dc.identifier.urihttp://hdl.handle.net/1828/14667
dc.language.isoenen_US
dc.publisherScientific Reportsen_US
dc.subjectCell biology
dc.subjectPhysiology
dc.subjectReproductive biology
dc.subject.departmentDivision of Medical Sciences
dc.subject.departmentSchool of Medical Sciences
dc.titleA 3D analysis revealed complexe mitochondria morphologies in porcine cumulus cellsen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Tremblay_Marie-Eve_SciRep_2022.pdf
Size:
2.06 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Faculty and Staff Publications
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.