Quantification of a COVID-19 antibody assay using a lateral flow test and a cell phone

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dc.contributor.authorThompson, Pearl
dc.contributor.authorBovolato, Ana Lívia de Carvalho
dc.contributor.authorIbáñez-Redín, Gisela
dc.contributor.authorBrolo, Alexandre G.
dc.date.accessioned2022-11-02T21:14:29Z
dc.date.available2022-11-02T21:14:29Z
dc.date.copyright2022en_US
dc.date.issued2022
dc.description.abstractAlthough several biomedical assays have been developed to screen for antibodies against SARS-CoV-2, very few can be completed without drawing blood. We developed a rapid lateral flow screening tool that used saliva samples and yielded rapid results that could be quantified using a cell phone. This assay provided the sensitive detection of IgG antibodies against SARS-CoV-2 within 10 min. We started by synthesising, modifying, and characterising gold nanoparticles. Using these particles as a coloured label, we developed a lateral flow strip made of nitrocellulose, glass fibre, and cellulose material. We quantified our visual results using pictures acquired with a cell phone and calculated a limit of detection of 4 ng/mL of antibodies against the SARS-CoV-2 spike protein.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis research was funded by Natural Sciences and Engineering Research Council (NSERC), CFI, BCKDF, and the University of Victoria, Department of Chemistry. Researchers received the NSERC Discovery Grant (#2020-04236) and NSERC Alliance COVID-19 grant (#550059-29).en_US
dc.identifier.citationThompson, P., Bovolato, A., Ibáñez-Redín, G., & Brolo, A. (2022). “Quantification of a COVID-19 antibody assay using a lateral flow test and a cell phone.” Chemosensors, 10(7), 234. https://doi.org/10.3390/chemosensors10070234en_US
dc.identifier.urihttps://doi.org/10.3390/chemosensors10070234
dc.identifier.urihttp://hdl.handle.net/1828/14384
dc.language.isoenen_US
dc.publisherChemosensorsen_US
dc.subjectlateral flow
dc.subjectsaliva
dc.subjectCOVID-19
dc.subjectIgG
dc.subjectSARS-CoV-2
dc.subjectgold nanoparticles
dc.subject.departmentDepartment of Chemistry
dc.titleQuantification of a COVID-19 antibody assay using a lateral flow test and a cell phoneen_US
dc.typeArticleen_US

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