Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses

Date

2018

Authors

Nieuwenhove, Nicolas Van
Potvin, Éric
Heikkilä, Maija
Pospelova, Vera
Mertens, Kenneth Neil
Masure, Edwige
Kucharska, Małgorzata
Yang, Eun Jin
Chomérat, Nicolas
Zajaczkowski, Marek

Journal Title

Journal ISSN

Volume Title

Publisher

Palynology

Abstract

We restudied the morphological complex comprising the cyst-based species Spiniferites elongatus/Spiniferites frigidus/Rottnestia amphicavata. We reviewed existing studies, and acquired new morphometric measurements of recent cysts from across the Northern Hemisphere, scanning electron microscopy (SEM) observations of cysts from Barents Sea surface sediments, and genetic analyses of cysts from the Beaufort Sea. The measurements suggest that populations and morphospecies cannot be distinguished based on morphometric criteria. Furthermore, sequential sediment trap samples from Hudson Bay reveal that morphological variation can occur at the same location over a few weeks, arguing against a uniform morphological response to environmental parameters. The SEM observations reveal a consistent gonyaulacacean tabulation (Po, 4′, 6′′, 6c, 5 s, *6′′′, 1p, 1′′′′). The small and large subunit ribosomal RNA genes and the internal transcribed spacer sequences obtained from differing cysts from the Beaufort Sea with morphologies attributable to the complex, including forms that correspond to Rottnestia amphicavata, were all identical and conform to those of Gonyaulax elongata from the Orkney Islands. The molecular analyses thus support the conclusion that the morphological variability is not reflected genetically and occurs within one species. Based on arguments against the generic attribution of amphicavata to Rottnestia, the continuum between the extreme ends of the morphological range, and the molecular data, we suggest Rottnestia amphicavata to be conspecific with Spiniferites frigidus, and both to be junior synonyms of Spiniferites elongatus. The morphometric data further indicate that Spiniferites ellipsoideus, an elongate cyst from the Miocene, can also be considered a junior synonym of Spiniferites elongatus. It is recommended to use two informal types in census work (i.e. Spiniferites elongatus – Beaufort morphotype for morphologies formerly assignable to Spiniferites frigidus/Rottnestia amphicavata, and Spiniferites elongatus – Norwegian morphotype for cysts with strongly reduced processes) to separate specimens at both extreme ends of the morphological spectrum from typical specimens of Spiniferites elongatus.

Description

Keywords

single-cell PCR, morphologic variability, taxonomy

Citation

Nieuwenhove, N. V., Potvin, E., Heikkilä, M., Pospelova, V., Mertens, K. N., Masure, E. …Zajaczkowski, M. (2018). Taxonomic revision of Spiniferites elongatus (the resting stage of Gonyaulax elongata) based on morphological and molecular analyses. Palynology, 42(1), 111-134. https://doi.org/10.1080/01916122.2018.1465736