3D Bioprinting Human Induced Pluripotent Stem Cell-Derived Neural Tissues Using a Novel Lab-on-a-Printer Technology

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dc.contributor.authorDe la Vega, Laura
dc.contributor.authorGómez, Diego A. Rosas
dc.contributor.authorAbelseth, Emily
dc.contributor.authorAbelseth, Laila
dc.contributor.authorAllisson da Silva, Victor
dc.contributor.authorWillerth, Stephanie M.
dc.date.accessioned2019-01-24T20:34:52Z
dc.date.available2019-01-24T20:34:52Z
dc.date.copyright2018en_US
dc.date.issued2018-11
dc.description.abstractMost neurological diseases and disorders lack true cures, including spinal cord injury (SCI). Accordingly, current treatments only alleviate the symptoms of these neurological diseases and disorders. Engineered neural tissues derived from human induced pluripotent stem cells (hiPSCs) can serve as powerful tools to identify drug targets for treating such diseases and disorders. In this work, we demonstrate how hiPSC-derived neural progenitor cells (NPCs) can be bioprinted into defined structures using Aspect Biosystems’ novel RX1 bioprinter in combination with our unique fibrin-based bioink in rapid fashion as it takes under 5 min to print four tissues. This printing process preserves high levels of cell viability (>81%) and their differentiation capacity in comparison to less sophisticated bioprinting methods. These bioprinted neural tissues expressed the neuronal marker, βT-III (45 ± 20.9%), after 15 days of culture and markers associated with spinal cord (SC) motor neurons (MNs), such as Olig2 (68.8 ± 6.9%), and HB9 (99.6 ± 0.4%) as indicated by flow cytometry. The bioprinted neural tissues expressed the mature MN marker, ChaT, after 30 days of culture as indicated by immunocytochemistry. In conclusion, we have presented a novel method for high throughput production of mature hiPSC-derived neural tissues with defined structures that resemble those found in the SC.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was funded by the British Columbia Innovation Council’s Ignite award, the NSERC Discovery Grants program, and the Canada Research Chairs programs.en_US
dc.identifier.citationDe la Vega, L., Rosas Gómez, Diego A., Abelseth, E., Abelseth, L., Allisson da Silva, V. & Willerth, S.M. (2018). 3D Bioprinting Human Induced Pluripotent Stem Cell- Derived Neural Tissues Using a Novel Lab-on-a-Printer Technology. Applied Sciences, 8(12), 2414. https://doi.org/10.3390/app8122414en_US
dc.identifier.urihttps://doi.org/10.3390/app8122414
dc.identifier.urihttp://hdl.handle.net/1828/10550
dc.language.isoenen_US
dc.publisherApplied Sciencesen_US
dc.subject3D bioprintingen_US
dc.subjectneural tissueen_US
dc.subjectmotor neuronsen_US
dc.subjectpluripotent stem cellsen_US
dc.subjectbiomaterialsen_US
dc.subjectspinal cord injuryen_US
dc.subjectlab on a printeren_US
dc.subjectfibrinen_US
dc.title3D Bioprinting Human Induced Pluripotent Stem Cell-Derived Neural Tissues Using a Novel Lab-on-a-Printer Technologyen_US
dc.typeArticleen_US

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