Electrospun biomaterial scaffolds with varied topographies for neuronal differentiation of human induced pluripotent stem cells

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dc.contributor.authorMohtaram, Nima Khadem
dc.contributor.authorKo, Junghyuk
dc.contributor.authorKing, Craig
dc.contributor.authorSun, Lin
dc.contributor.authorMuller, Nathan
dc.contributor.authorJun, Martin Byung-Guk
dc.contributor.authorWillerth, Stephanie
dc.date.accessioned2015-07-03T16:57:07Z
dc.date.available2016-01-03T12:22:05Z
dc.date.copyright2014en_US
dc.date.issued2014-12-30
dc.descriptionPreprint article.en_US
dc.description.abstractIn this study, we investigated the effect of micro and nanoscale scaffold topography on promoting neuronal differentiation of human induced pluripotent stem cells (iPSCs) and directing the resulting neuronal outgrowth in an organized manner. We used melt electrospinning to fabricate poly (ε-caprolactone) (PCL) scaffolds with loop mesh and biaxial aligned microscale topographies. Biaxial aligned microscale scaffolds were further functionalized with retinoic acid releasing PCL nanofibers using solution electrospinning. These scaffolds were then seeded with neural progenitors derived from human iPSCs. We found that smaller diameter loop mesh scaffolds (43.7 ± 3.9 μm) induced higher expression of the neural markers Nestin and Pax6 compared to thicker diameter loop mesh scaffolds (85 ± 4 μm). The loop mesh and biaxial aligned scaffolds guided the neurite outgrowth of human iPSCs along the topographical features with the maximum neurite length of these cells being longer on the biaxial aligned scaffolds. Finally, our novel bimodal scaffolds also supported the neuronal differentiation of human iPSCs as they presented both physical and chemical cues to these cells, encouraging their differentiation. These results give insight into how physical and chemical cues can be used to engineer neural tissue.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe authors would like to acknowledge the funding support from the Natural Sciences and Engineering Research Council’s Discovery Grants program.en_US
dc.identifier.citationMohtaram N.K. et al. (2015) Electrospun biomaterial scaffolds with varied topographies for neuronal differentiation of human induced pluripotent stem cells. Journal of Biomedical Materials Research. Part A. 103 (8) p.2591-2601.en_US
dc.identifier.urihttp://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35392/abstract
dc.identifier.urihttp://hdl.handle.net/1828/6285
dc.language.isoenen_US
dc.publisherWileyen_US
dc.subjecthuman induced pluripotent stem cells
dc.subjectmelt electrospinning
dc.subjectsolution electrospinning
dc.subjectscaffold topography
dc.subjectneural tissue engineering
dc.subject.departmentDepartment of Mechanical Engineering
dc.subject.departmentSchool of Medical Sciences
dc.titleElectrospun biomaterial scaffolds with varied topographies for neuronal differentiation of human induced pluripotent stem cellsen_US
dc.typePreprinten_US

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