Growth conditions and freeze/thaw survival of Chinese hamster ovary cells
Date
1979
Authors
Goshko, Susanne Lynn
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Abstract
The survival of CHO cells in tissue culture after freezing and thawing was assayed after altering the fatty acid constituents and the cholesterol content of the plasma membranes.
Only when cholesterol concentration has been increased in the membrane was survival altered at the slowest cooling rate of 1°C/minute. Survival at other cooling rates was unchanged.
Changing the fatty acid constituents in the membrane, as assayed biologically , had no effect on freeze/thaw survival. However, addition of avidin, a glycoprotein which binds biotin, significantly increased freeze/thaw survival at the slow cooling rate of 1°C/minute.