Growth conditions and freeze/thaw survival of Chinese hamster ovary cells

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dc.contributor.authorGoshko, Susanne Lynnen_US
dc.date.accessioned2024-08-13T22:56:25Z
dc.date.available2024-08-13T22:56:25Z
dc.date.copyright1979en_US
dc.date.issued1979
dc.degree.departmentDepartment of Biology
dc.degree.levelMaster of Science M.Sc.en
dc.description.abstractThe survival of CHO cells in tissue culture after freezing and thawing was assayed after altering the fatty acid constituents and the cholesterol content of the plasma membranes. Only when cholesterol concentration has been increased in the membrane was survival altered at the slowest cooling rate of 1°C/minute. Survival at other cooling rates was unchanged. Changing the fatty acid constituents in the membrane, as assayed biologically , had no effect on freeze/thaw survival. However, addition of avidin, a glycoprotein which binds biotin, significantly increased freeze/thaw survival at the slow cooling rate of 1°C/minute.en_US
dc.format.extent193 pages
dc.identifier.urihttps://hdl.handle.net/1828/17966
dc.rightsAvailable to the World Wide Weben_US
dc.titleGrowth conditions and freeze/thaw survival of Chinese hamster ovary cellsen_US
dc.typeThesisen_US

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