High temporal-resolution view of transcription and chromatin states across distinct metabolic states in budding yeast
| dc.contributor.author | Kuang, Z. | |
| dc.contributor.author | Cai, L. | |
| dc.contributor.author | Zhang, Xuekui | |
| dc.contributor.author | Ji, H. | |
| dc.contributor.author | Tu, B.P. | |
| dc.contributor.author | Boeke, J.D. | |
| dc.date.accessioned | 2021-08-18T19:06:19Z | |
| dc.date.available | 2021-08-18T19:06:19Z | |
| dc.date.copyright | 2014 | en_US |
| dc.date.issued | 2014 | |
| dc.description.abstract | Under continuous, glucose-limited conditions, budding yeast exhibit robust metabolic cycles associated with major oscillations of gene expression. How such fluctuations are linked to changes in chromatin status is not well understood. Here we examine the correlated genome-wide transcription and chromatin states across the yeast metabolic cycle at unprecedented temporal resolution, revealing a “just-in-time supply chain” by which components from specific cellular processes such as ribosome biogenesis become available in a highly coordinated manner. We identify distinct chromatin and splicing patterns associated with different gene categories and determine the relative timing of chromatin modifications to maximal transcription. There is unexpected variation in the chromatin modification and expression relationship, with histone acetylation peaks occurring with varying timing and “sharpness” relative to RNA expression both within and between cycle phases. Chromatin modifier occupancy reveals subtly distinct spatial and temporal patterns compared to the modifications themselves. | en_US |
| dc.description.reviewstatus | Reviewed | en_US |
| dc.description.scholarlevel | Faculty | en_US |
| dc.description.sponsorship | This work was supported by NIH grants R01HG006841 to H.J., R01GM094314 to B.P.T. and U54GM103520 to J.D.B. | en_US |
| dc.identifier.citation | Kuang, Z., Cai, L., Zhang, X., Ji, H., Tu, B.P., Boeke, J.D. (2014). High temporalresolution view of transcription and chromatin states across distinct metabolic states in budding yeast. Nature Structural & Molecular Biology, 21, 854-863. https://doi.org/10.1038/nsmb.2881 | en_US |
| dc.identifier.uri | https://doi.org/10.1038/nsmb.2881 | |
| dc.identifier.uri | http://hdl.handle.net/1828/13282 | |
| dc.language.iso | en | en_US |
| dc.publisher | Nature Structural & Molecular Biology | en_US |
| dc.subject.department | Department of Mathematics and Statistics | |
| dc.title | High temporal-resolution view of transcription and chromatin states across distinct metabolic states in budding yeast | en_US |
| dc.type | Article | en_US |