Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar

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dc.contributor.authorDafoe, Nicole J.
dc.contributor.authorGowen, Brent E.
dc.contributor.authorConstabel, C. Peter
dc.date.accessioned2014-07-28T22:58:26Z
dc.date.available2014-07-28T22:58:26Z
dc.date.copyright2010en_US
dc.date.issued2010-08-26
dc.descriptionBioMed Centralen_US
dc.description.abstractBackground: Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (Populus trichocarpa × P. deltoides) using proteomics methods, and their sieve element localization confirmed by immunofluorescence. In the current study, we analyzed different tissues to further understand TLP expression and localization in poplar, and used immunogold labelling to determine intracellular localization. Results: Immunofluorescence using a TLP antiserum confirmed the presence of TLP in punctate, organelle-like structures within sieve elements. On western blots, the antiserum labeled two constitutively expressed proteins with distinct expression patterns. Immunogold labelling suggested that TLPs are associated with starch granules and starch-containing plastids in sieve elements and phloem parenchyma cells. In addition, the antiserum recognized TLPs in the inner cell wall and sieve plate region of sieve elements. Conclusions: TLP localization in poplar cells and tissues is complex. TLP1 is expressed predominantly in tissues with a prominent vascular system such as midveins, petioles and stems, whereas the second TLP is primarily expressed in starch-storing plastids found in young leaves and the shoot apex.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipWe gratefully acknowledge the support of the Natural Sciences and Engineering Research Council (NSERC) Canada in the form of Discovery Grants (to C.P.C) and the Pacific Forestry Centre of the Canadian Forest Service in the form of a Graduate Student Award (to N.D.).en_US
dc.identifier.citationDafoe et al.: Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar. BMC Plant Biology 2010 10:191.en_US
dc.identifier.urihttp://www.biomedcentral.com/1471-2229/10/191
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2229-10-191
dc.identifier.urihttp://hdl.handle.net/1828/5493
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.subjectCentre for Forest Biology
dc.subject.departmentDepartment of Biology
dc.titleThaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplaren_US
dc.typeArticleen_US

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