Raman spectroscopy identifies radiation response in human non-small cell lung cancer xenografts

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dc.contributor.authorHarder, Samantha J.
dc.contributor.authorIsabelle, Martin
dc.contributor.authorDeVorkin, Lindsay
dc.contributor.authorSmazynski, Julian
dc.contributor.authorBeckham, Wayne
dc.contributor.authorBrolo, Alexandre G.
dc.contributor.authorLum, Julian J.
dc.contributor.authorJirasek, Andrew
dc.date.accessioned2019-05-03T12:43:05Z
dc.date.available2019-05-03T12:43:05Z
dc.date.copyright2016en_US
dc.date.issued2016
dc.description.abstractExternal beam radiation therapy is a standard form of treatment for numerous cancers. Despite this, there are no approved methods to account for patient specific radiation sensitivity. In this report, Raman spectroscopy (RS) was used to identify radiation-induced biochemical changes in human non-small cell lung cancer xenografts. Chemometric analysis revealed unique radiation-related Raman signatures that were specific to nucleic acid, lipid, protein and carbohydrate spectral features. Among these changes was a dramatic shift in the accumulation of glycogen spectral bands for doses of 5 or 15 Gy when compared to unirradiated tumours. When spatial mapping was applied in this analysis there was considerable variability as we found substantial intra- and inter-tumour heterogeneity in the distribution of glycogen and other RS spectral features. Collectively, these data provide unique insight into the biochemical response of tumours, irradiated in vivo, and demonstrate the utility of RS for detecting distinct radiobiological responses in human tumour xenografts.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe authors wish to thank the Medical Physics team at the BC Cancer Agency Vancouver Island Centre and the VIC Animal Care Unit for their support and assistance. This work was supported by funding from CIHR (JJL, AGB, AJ), Cancer Research Society (JJL), Western Economic Diversification (AGB, AJ), CFI (AGB), NSERC-CGS-D (SJH), and the BC Cancer Foundation (JJL).en_US
dc.identifier.citationHarder, S.J., Isabelle, M., DeVorkin, L., Smazynski, J., Beckham, W., Brolo, A.G., Lum, J.J. & Jirasek, A. (2019). Raman spectroscopy identifies radiation response in human non-small cell lung cancer xenografts. Scientific Reports, 6: 21006. https://doi.org/10.1038/srep21006en_US
dc.identifier.urihttps://doi.org/10.1038/srep21006
dc.identifier.urihttp://hdl.handle.net/1828/10843
dc.language.isoenen_US
dc.publisherScientific Reportsen_US
dc.subject.departmentDepartment of Physics and Astronomy
dc.subject.departmentDepartment of Chemistry
dc.subject.departmentDepartment of Biochemistry and Microbiology
dc.titleRaman spectroscopy identifies radiation response in human non-small cell lung cancer xenograftsen_US
dc.typeArticleen_US

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