Pannexin 1 regulates postnatal neural stem and progenitor cell proliferation

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dc.contributor.authorWicki-Stordeur, Leigh E.
dc.contributor.authorDzugalo, Adrian D.
dc.contributor.authorSwansburg, Rose M.
dc.contributor.authorSuits, Jocelyne M.
dc.contributor.authorSwayne, Leigh Anne
dc.date.accessioned2014-07-21T18:27:59Z
dc.date.available2014-07-21T18:27:59Z
dc.date.copyright2012en_US
dc.date.issued2012-03-29
dc.descriptionBioMed Centralen_US
dc.description.abstractBackground: Pannexin 1 forms ion and metabolite permeable hexameric channels and is abundantly expressed in the brain. After discovering pannexin 1 expression in postnatal neural stem and progenitor cells we sought to elucidate its functional role in neuronal development. Results: We detected pannexin 1 in neural stem and progenitor cells in vitro and in vivo. We manipulated pannexin 1 expression and activity in Neuro2a neuroblastoma cells and primary postnatal neurosphere cultures to demonstrate that pannexin 1 regulates neural stem and progenitor cell proliferation likely through the release of adenosine triphosphate (ATP). Conclusions: Permeable to ATP, a potent autocrine/paracine signaling metabolite, pannexin 1 channels are ideally suited to influence the behavior of neural stem and progenitor cells. Here we demonstrate they play a robust role in the regulation of neural stem and progenitor cell proliferation. Endogenous postnatal neural stem and progenitor cells are crucial for normal brain health, and their numbers decline with age. Furthermore, these special cells are highly responsive to neurological injury and disease, and are gaining attention as putative targets for brain repair. Therefore, understanding the fundamental role of pannexin 1 channels in neural stem and progenitor cells is of critical importance for brain health and disease.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by a Natural Sciences and Engineering Research Council of Canada Discovery Grant, and a University of Victoria laboratory start-up grant awarded to LAS. We thank Jen Graham for assistance with mouse perfusions and decapitations, and we thank Dr. Robert Burke and the UVIC Department of Biochemistry and Microbiology for confocal microscope access.en_US
dc.identifier.citationWicki-Stordeur et al.: Pannexin 1 regulates postnatal neural stem and progenitor cell proliferation. Neural Development 2012 7:11.en_US
dc.identifier.urihttp://www.neuraldevelopment.com/content/7/1/11
dc.identifier.urihttp://dx.doi.org/10.1186/1749-8104-7-11
dc.identifier.urihttp://hdl.handle.net/1828/5477
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.subjectPostnatal neurogenesis
dc.subjectPannexin 1
dc.subjectNeural stem and progenitor cells
dc.subjectCell proliferation
dc.subjectATP release
dc.subjectIsland Medical Program
dc.subject.departmentDivision of Medical Sciences
dc.subject.departmentSchool of Medical Sciences
dc.subject.departmentDepartment of Biology
dc.subject.departmentDepartment of Biochemistry and Microbiology
dc.titlePannexin 1 regulates postnatal neural stem and progenitor cell proliferationen_US
dc.typeArticleen_US

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