Effects of medium supplements on somatic embryo maturation and DNA methylation in Pseudotsuga gaussenii Flous, a species under protection
Date
2022
Authors
Gao, Ying
Chen, Xiaoyi
Cui, Ying
Zhao, Huanhuan
Zhao, Ruirui
Liu, Chengbi
Zhao, Jian
Zhang, Jinfeng
Kong, Lisheng
Journal Title
Journal ISSN
Volume Title
Publisher
Forests
Abstract
Pseudotsuga gaussenii is a forest species under protection in China. Propagation via somatic
embryogenesis is efficient and needed for recovering this species. In this research, effects of medium
supplements, i.e., methylglyoxal-bis (guanylhydrazone) (MGBG), polyethylene glycol (PEG), and
maltose, on somatic embryo (SE) development were studied. More mature SEs developed with
the pre-maturation treatment medium supplemented with 50 M or 100 M MGBG. During SE
maturation, when the medium was supplemented with both PEG and maltose, the over-proliferation
of embryogenic tissue was suppressed. When maltose was eliminated from the maturation medium,
SEs were slightly smaller but developed better than other treatments due to the supply of lower
carbon source, whereas when PEG was taken out from the maturation medium, no mature SE was
obtained, only the heavily-proliferated plant tissue. Meanwhile, DNA methylation levels differed in
these SE maturation cultures at the late culture stage of SE maturation. The level increased sharply
with no maltose treatment (20.28%) while decreasing without PEG supplement (10.35%). DNA
methyltransferase genes MET1-1, MET1-2, MET1-3, CMT3, DRM1, and DRM2 were partially cloned
in this study to detect their expression level via qPCR. Expression of these genes, except MET1-3,
in the culture of no PEG was higher significantly than the cultures of other treatments at weeks 1,
3, and 10. Our results suggested that MGBG and/or PEG play an important role in stimulating
SE development and maturation. Furthermore, sugar supplements at a lower level benefited SE
maturation in Pseudotsuga gaussenii.
Description
Keywords
MCBG, PEG, maltose, embryogenic tissues, proliferation, maturation, DNA methyltransferase, conifer, Centre for Forest Biology
Citation
APA style