Incorporation of retinoic acid releasing microspheres into pluripotent stem cell aggregates for inducing neuronal differentiation

Simple item page
dc.contributor.authorGomez, Jose Carlos
dc.contributor.authorEdgar, John M
dc.contributor.authorAgbay, Andrew M
dc.contributor.authorBibault, Emma
dc.contributor.authorMontgomery, Amy
dc.contributor.authorMohtaram, Nima Khadem
dc.contributor.authorWillerth, Stephanie
dc.date.accessioned2015-06-15T23:11:32Z
dc.date.available2016-06-05T11:22:06Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.description.abstractPluripotent stem cells (PSCs) can form any specialized cell type found in the body making them an excellent tool for regenerative medicine applications. Directed differentiation of PSCs into specific phenotypes can be accomplished by introducing specific chemical cues such as the small molecule retinoic acid (RA). Expressed in the developing nervous system, RA can induce differentiation of PSCs into neural phenotypes including neurons. In this study, we encapsulated all-trans RA within poly (ɛ-caprolactone) (PCL) microspheres to generate controlled morphogen release over 28 days. RA/PCL microspheres less than ~10 μm in diameter were readily incorporated within the interstitial sites of human induced pluripotent stem cell (hiPSC) aggregates. After 5 days of culture, the microspheres did not induce cytotoxic effects and the hiPSC aggregates containing microspheres showed a decrease in the pluripotency marker SSEA-4. After 7 days of culture on laminin surfaces, aggregates expressed the neuronal marker TUJ1 and displayed extended neurite outgrowth. This approach provides consistent RA delivery throughout the aggregate and could be an effective strategy for differentiating cells in vivo. Overall, our results demonstrate that it is possible to combine hiPSC aggregates with RA/PCL microspheres for neural tissue engineering applications.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe authors would like to thank funding from the University of Victoria, the Canada Research Chairs program, the Rick Hansen Foundation, and the Natural Science and Engineering Research Council for funding for this work.en_US
dc.identifier.citationGomez et al. Incorporation of retinoic acid releasing microspheres into aggregates of pluripotent stem cells for inducing neuronal differentiation. In press at Cellular and Molecular Bioengineering.en_US
dc.identifier.urihttp://link.springer.com/article/10.1007/s12195-015-0401-z
dc.identifier.urihttp://hdl.handle.net/1828/6263
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.subjectcontrolled release
dc.subjectdrug delivery
dc.subjectneural tissue engineering
dc.subjectpoly(ɛ-caprolactone)
dc.subjectembryoid body
dc.subject.departmentDepartment of Mechanical Engineering
dc.subject.departmentSchool of Medical Sciences
dc.titleIncorporation of retinoic acid releasing microspheres into pluripotent stem cell aggregates for inducing neuronal differentiationen_US
dc.typePreprinten_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Gomez_Jose_CellMolBioengn_preprint_2015.pdf
Size:
1.64 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.74 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Faculty and Staff Publications
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.