Evolutionary classification of ammonium, nitrate, and peptide transporters in land plants

Simple item page
dc.contributor.authorVon Wittgenstein, Neil JJB
dc.contributor.authorLe, Cuong H
dc.contributor.authorHawkins, Barbara J.
dc.contributor.authorEhlting, Jürgen
dc.date.accessioned2015-06-02T21:06:52Z
dc.date.available2015-06-02T21:06:52Z
dc.date.copyright2014en_US
dc.date.issued2014-01-20
dc.descriptionBioMed Centralen_US
dc.description.abstractBackground: Nitrogen uptake, reallocation within the plant, and between subcellular compartments involves ammonium, nitrate and peptide transporters. Ammonium transporters are separated into two distinct families (AMT1 and AMT2), each comprised of five members on average in angiosperms. Nitrate transporters also form two discrete families (NRT1 and NRT2), with angiosperms having four NRT2s, on average. NRT1s share an evolutionary history with peptide transporters (PTRs). The NRT1/PTR family in land plants usually has more than 50 members and contains also members with distinct activities, such as glucosinolate and abscisic acid transport. Results: Phylogenetic reconstructions of each family across 20 land plant species with available genome sequences were supplemented with subcellular localization and transmembrane topology predictions. This revealed that both AMT families diverged prior to the separation of bryophytes and vascular plants forming two distinct clans, designated as supergroups, each. Ten supergroups were identified for the NRT1/PTR family. It is apparent that nitrate and peptide transport within the NRT1/PTR family is polyphyletic, that is, nitrate and/or peptide transport likely evolved multiple times within land plants. The NRT2 family separated into two distinct clans early in vascular plant evolution. Subsequent duplications occurring prior to the eudicot/monocot separation led to the existence of two AMT1, six AMT2, 31 NRT1/PTR, and two NRT2 clans, designated as groups. Conclusion: Phylogenetic separation of groups suggests functional divergence within the angiosperms for each family. Distinct groups within the NRT1/PTR family appear to separate peptide and nitrate transport activities as well as other activities contained within the family, for example nitrite transport. Conversely, distinct activities, such as abscisic acid and glucosinolate transport, appear to have recently evolved from nitrate transporters.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by individual Discovery Grants from the Natural Sciences and Engineering Research Council of Canada (NSERC) to JE and BJH. NJJBvW and CHL received graduate stipends from the NSERC Collaborative Research and Training Experience Program (CREATE) in Forests and Climate Change.en_US
dc.identifier.citationvon Wittgenstein et al.: Evolutionary classification of ammonium, nitrate, and peptide transporters in land plants. BMC Evolutionary Biology 2014 14:11.en_US
dc.identifier.urihttp://www.biomedcentral.com/1471-2148/14/11
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2148-14-11
dc.identifier.urihttp://hdl.handle.net/1828/6219
dc.language.isoenen_US
dc.publisherBMC Evolutionary Biologyen_US
dc.rightsAttribution-NonCommercial-NoDerivs 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ca/*
dc.subjectAmmonium transporter (AMT1 and AMT2)
dc.subjectNitrate transporter (NRT1 and NRT2)
dc.subjectPeptide transporter (PTR)
dc.subjectGene family evolution
dc.subjectCentre for Forest Biology
dc.subject.departmentDepartment of Biology
dc.subject.departmentDepartment of Biochemistry and Microbiology
dc.titleEvolutionary classification of ammonium, nitrate, and peptide transporters in land plantsen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
vonWittgenstein_Neil_BMCEvolBiol_2014.pdf
Size:
3.57 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.74 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Faculty and Staff Publications
Publications (Centre for Forest Biology)
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.