A microfluidic toolbox for cell fusion

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dc.contributor.authorChiu, Flora W.Y.
dc.contributor.authorBagci, Hakan
dc.contributor.authorFisher, Amanda G.
dc.contributor.authordeMello, Andrew J.
dc.contributor.authorElvira, Katherine S.
dc.date.accessioned2018-11-02T08:16:35Z
dc.date.available2018-11-02T08:16:35Z
dc.date.copyright2016en_US
dc.date.issued2016
dc.description.abstractCellular fusion is a key process in many fields ranging from historical gene mapping studies and monoclonal antibody production, through to cell reprogramming. Traditional methodologies for cell fusion rely on the random pairing of different cell types and generally result in low and variable fusion efficiencies. These approaches become particularly limiting where substantial numbers of bespoke one-to-one fusions are required, for example, for in-depth studies of nuclear reprogramming mechanisms. In recent years, microfluidic technologies have proven valuable in creating platforms where the manipulation of single cells is highly efficient, rapid and controllable. These technologies also allow the integration of different experimental steps and characterisation processes into a single platform. Although the application of microfluidic methodologies to cell fusion studies is promising, current technologies that rely on static trapping are limited both in terms of the overall number of fused cells produced and their experimental accessibility. Here we review some of t he most exciting breakthroughs in core microfluidic technologies that will allow the creation of integrated platforms for controlled cell fusion at high throughput.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was partially (H. B and A. G. F.) supported by a European Research Council grant (#294627).en_US
dc.identifier.citationChiu, F.W.Y., Bagci, H., Fisher, A.G., deMello, A.J. & Elvira, K.S. (2016). A microfluidic toolbox for cell fusion. Journal of Chemical Technology & Biotechnology, 91(1), 16-24. https://doi.org/10.1002/jctb.4803en_US
dc.identifier.urihttps://doi.org/10.1002/jctb.4803
dc.identifier.urihttp://hdl.handle.net/1828/10232
dc.language.isoenen_US
dc.publisherJournal of Chemical Technology and Biotechnologyen_US
dc.subjectdroplet microfluidics
dc.subjectcell fusion
dc.subjectcell encapsulation
dc.subjectsingle-cell
dc.subjecthigh efficiency
dc.subjectcell sorting
dc.subject.departmentDepartment of Chemistry
dc.titleA microfluidic toolbox for cell fusionen_US
dc.typePostprinten_US

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