Expression and purification of the full murine NPM2 and study of its interaction with protamines and histones

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dc.contributor.authorEllard, Katherine
dc.contributor.authorSerpa, Jason J.
dc.contributor.authorPetrotchenko, Evgeniy V.
dc.contributor.authorBorchers, Christoph H.
dc.contributor.authorAusiĆ³, Juan
dc.date.accessioned2018-03-26T21:00:02Z
dc.date.available2018-03-26T21:00:02Z
dc.date.copyright2016en_US
dc.date.issued2016
dc.description.abstractMouse nucleoplasmin M.NPM2 was recombinantly expressed and the protein consisting of the complete sequence was purified and characterized. Similar to its Xenopus laevis X.NPM2 counterpart, the protein forms stable pentameric complexes and exhibits an almost undistinguishable hydrodynamic ionic strength-dependent unfolding behavior. The interaction of N.PM2 with histones and mouse P1/P2 protamines revealed that these chromosomal proteins bind preferentially to the distal part of the nucleoplasmin pentamer. Moreover, the present work highlights the critical role played by histones H2B and H4 in the association of the histone H2A-H2B dimers and histone octamer with nucleoplasmin.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by a Natural Sciences and Engineering Research Council of Canada (NSERC) 46399-2012 grant to J.A. CHB would like to thank for support from the the Genomic Innovations Network (GIN) from Genome Canada and Genome British Columbia, and the National Sciences and Engineering Research Council of Canada for RGPIN 346194-2013 grant support.en_US
dc.identifier.citationEllard, K., Serpa, J.J., Petrotchenko, E.V., Borchers, C.H. & AusiĆ³, J. (2016). Expression and purification of the full murine NPM2 and study of its interaction with protamines and histones. Biochemistry and Biophysics Reports 6, 165-171. http://dx.doi.org/10.1016/j.bbrep.2016.04.002en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.bbrep.2016.04.002
dc.identifier.urihttp://hdl.handle.net/1828/9166
dc.language.isoenen_US
dc.publisherBiochemistry andBiophysicsReportsen_US
dc.subjectUVic Genome BC Proteomics Centre
dc.subject.departmentDepartment of Biochemistry and Microbiology
dc.titleExpression and purification of the full murine NPM2 and study of its interaction with protamines and histonesen_US
dc.typeArticleen_US

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