Ions, metabolites, and cells: Water as a reporter of surface conditions during bacterial growth

Simple item page
dc.contributor.authorJarisz, Tasha A.
dc.contributor.authorLane, Sarah
dc.contributor.authorGozdzialski, Lea
dc.contributor.authorHore, Dennis K.
dc.date.accessioned2019-12-17T23:59:02Z
dc.date.available2019-12-17T23:59:02Z
dc.date.copyright2018en_US
dc.date.issued2018
dc.description.abstractSurface-specific nonlinear vibrational spectroscopy, combined with bulk solution measurements and imaging, is used to study the surface conditions during the growth of E. coli. As a result of the silica high surface charge density, the water structure at the silica–aqueous interface is known to be especially sensitive to pH and ionic strength, and surface concentration profiles develop that can be appreciably different from the bulk solution conditions. We illustrate that, in the presence of growing cells, a unique surface micro-environment is established as a result of metabolites accumulating on the silica surface. Even in the subsequent absence of the cells, this surface layer works to reduce the interfacial ionic strength as revealed by the enhanced signal from surface water molecules. In the presence of growing cells, an additional boost in surface water signal is attributed to a local pH that is higher than that of the bulk solution.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis project was supported by a Discovery Grant from the Natural Science and Engineering Research Council of Canada (NSERC). The SFG spectrometer was purchased with assistance from the Canadian Foundation for Innovation Leader’s Opportunity Fund and the British Columbia Knowledge Development Fund. We thank Sarah Kowallik, Adrianna Thompson, and Nat Jafarova for assistance with the cell surface adhesion characterization. Barb Currie and Rebecca Hof (UVic Biochemistry and Microbiology) provided valuable advice on the cell growth and preparation procedures. We thank Dr. Elizabeth Schneider at Biolin Scientific for use of the QCM-D instrument at the University of Victoria. Dr. Matthew Dixon at Biolin Scientific provided assistance with the QCM-D data analysis. T.A.J. is grateful to NSERC for a CGS-M scholarship. L.G. has received an NSERC USRA scholarship.en_US
dc.identifier.citationJarisz, T. A.; Lane, S.; Gozdzialski, L.; & Hore, D. K. (2018). Ions, metabolites, and cells: Water as a reporter of surface conditions during bacterial growth. The Journal of Chemical Physics, 148, 222825. DOI: 10.1063/1.5023748en_US
dc.identifier.urihttps://doi.org/10.1063/1.5023748
dc.identifier.urihttp://hdl.handle.net/1828/11376
dc.language.isoenen_US
dc.publisherThe Journal of Chemical Physicsen_US
dc.subjectOptical properties
dc.subjectVibrational spectroscopy
dc.subjectBacteria
dc.subjectChemical elements
dc.subjectBacterial growth
dc.subjectCell cultures
dc.subjectProteins
dc.subjectHydrology
dc.subjectAqueous interfaces
dc.subjectCell growth
dc.subject.departmentDepartment of Chemistry
dc.titleIons, metabolites, and cells: Water as a reporter of surface conditions during bacterial growthen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Jarisz_Tasha_JChemPhys_2018.pdf
Size:
1.81 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Faculty and Staff Publications
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.