In vitro and in vivo evaluation of silk fibroin-hardystonite-gentamicin nanofibrous scaffold for tissue engineering applications
Date
2020
Authors
Hadisi, Zhina
Bakhsheshi-Rad, Hamid Reza
Walsh, Tavia
Dehghan, Mohammad Mehdi
Farzad-Mohajeri, Saeed
Gholami, Hossein
Diyanoush, Anahita
Pagan, Erik
Journal Title
Journal ISSN
Volume Title
Publisher
Polymer Testing
Abstract
Designing advanced biomaterials with regenerative and drug delivering functionalities remains a challenge in
the field of tissue engineering. In this paper we present the design, development, and a use case of an electrospun
nano-biocomposite scaffold composed of silk fibroin (SF), hardystonite (HT), and gentamicin (GEN). The
fabricated SF nanofiber scaffolds provide mechanical support while HT acts as a bioactive and drug carrier, on
which GEN is loaded as an antibacterial agent. Antibacterial zone of inhibition (ZOI) results indicate that the
inclusion of 3–6 wt% GEN significantly improves the antibacterial performance of the scaffolds against Gramnegative
Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) bacteria, with an initial
burst release of 10–20% and 72–85% total release over 7 days. The release rate of stimulatory silicon ions from
SF-HT scaffolds reached 94.53±5 ppm after 7 days. Cell studies using osteoblasts show that the addition of HT
significantly improved the cytocompatibility of the scaffolds. Angiogenesis, in vivo biocompatibility, tissue
vascularization, and translatability of the scaffolds were studied via subcutaneous implantation in a rodent model
over 4-weeks. When implanted subcutaneously, the GEN-loaded scaffold promoted angiogenesis and collagen
formation, which suggests that the scaffold may be highly beneficial for further bone tissue engineering
applications.
Description
Keywords
Drug delivery, In vivo, Subcutaneous implant, Nanofiber, Electrospinning, Silk fibroin, Laboratory for Innovations in Micro Engineering (LiME), Centre for Advanced Materials and Related Technology (CAMTEC)
Citation
Hadisi, Z., Bakhsheshi-Rad, H. R., Walsh, T., Dehghan, M. M., Farzad-Mohajeri, S., Gholami, H., … Akbari, M. (2020). In vitro and in vivo evaluation of silk fibroinhardystonite- gentamicin nanofibrous scaffold for tissue engineering applications. Polymer Testing, 91, 1-7. https://doi.org/10.1016/j.polymertesting.2020.106698.