Development of an integrated microfluidic platform to evaluate radiotherapy response of tumour cells




Palacios Sánchez, América

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This thesis details the design, fabrication, and testing of two optofluidic platforms, a square fused silica capillary and a MgF2-PDMS microfluidic chip to detect radiation-induced biochemical changes in cells during radiation treatment (radiotherapy). The platforms integrate a near-infrared Raman system of 785 nm excitation and a fiber-based optical trap at 1064 nm in a dual-beam configuration for the manipulation and subsequent examination of single polystyrene beads (5µm) and two breast carcinoma cell lines, MCF-7, and MDA-MB-23 (20-30 µm). Particular attention was paid to the role of MgF2 as a novel substrate for microfluidic fabrication and the device background contributions that could hinder spectral contributions from the samples. Successful optical trapping within the platforms was performed, which allowed the sample immobilization for the entire Raman acquisition time (10-30 s) via an orthogonally positioned objective for the excitation and collection of Raman signal. Data collected in the MgF2-PDMS microchip yielded high-quality spectra with no presence of PDMS characteristic Raman peaks in the spectral region of 450-1800 cm-1.



Radiotherapy, Microfluidics, Optofluidics, Cancer, Cancer cells, Optical trapping, MgF2, Microfabrication, Microchip, Raman