Development of an integrated microfluidic platform to evaluate radiotherapy response of tumour cells

Simple item page
dc.contributor.authorPalacios Sánchez, América
dc.contributor.supervisorBrolo, Alexandre Guimaraes
dc.date.accessioned2022-05-03T00:19:37Z
dc.date.copyright2022en_US
dc.date.issued2022-05-02
dc.degree.departmentDepartment of Chemistry
dc.degree.levelMaster of Science M.Sc.en_US
dc.description.abstractThis thesis details the design, fabrication, and testing of two optofluidic platforms, a square fused silica capillary and a MgF2-PDMS microfluidic chip to detect radiation-induced biochemical changes in cells during radiation treatment (radiotherapy). The platforms integrate a near-infrared Raman system of 785 nm excitation and a fiber-based optical trap at 1064 nm in a dual-beam configuration for the manipulation and subsequent examination of single polystyrene beads (5µm) and two breast carcinoma cell lines, MCF-7, and MDA-MB-23 (20-30 µm). Particular attention was paid to the role of MgF2 as a novel substrate for microfluidic fabrication and the device background contributions that could hinder spectral contributions from the samples. Successful optical trapping within the platforms was performed, which allowed the sample immobilization for the entire Raman acquisition time (10-30 s) via an orthogonally positioned objective for the excitation and collection of Raman signal. Data collected in the MgF2-PDMS microchip yielded high-quality spectra with no presence of PDMS characteristic Raman peaks in the spectral region of 450-1800 cm-1.en_US
dc.description.embargo2023-04-08
dc.description.scholarlevelGraduateen_US
dc.identifier.urihttp://hdl.handle.net/1828/13940
dc.languageEnglisheng
dc.language.isoenen_US
dc.rightsAvailable to the World Wide Weben_US
dc.subjectRadiotherapyen_US
dc.subjectMicrofluidicsen_US
dc.subjectOptofluidicsen_US
dc.subjectCanceren_US
dc.subjectCancer cellsen_US
dc.subjectOptical trappingen_US
dc.subjectMgF2en_US
dc.subjectMicrofabricationen_US
dc.subjectMicrochipen_US
dc.subjectRamanen_US
dc.titleDevelopment of an integrated microfluidic platform to evaluate radiotherapy response of tumour cellsen_US
dc.typeThesisen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Palacios_America_MSc_2022 .pdf
Size:
3.51 MB
Format:
Adobe Portable Document Format
Description:
Thesis
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Electronic Theses and Dissertations (ETD)
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.