Host–parasite transcriptomics during immunostimulant-enhanced rejection of salmon lice (Lepeophtheirus salmonis) by Atlantic salmon (Salmo salar)

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dc.contributor.authorSutherland, Ben J.G.
dc.contributor.authorCovello, Jennifer M.
dc.contributor.authorFriend, Sarah E.
dc.contributor.authorPoley, Jordan D.
dc.contributor.authorKoczka, Kim W.
dc.contributor.authorPurcell, Sara L.
dc.contributor.authorMacLeod, Tara L.
dc.contributor.authorDonovan, Bridget R.
dc.contributor.authorPino, Jorge
dc.contributor.authorGonzález-Vecino, Jose Luis
dc.contributor.authorGonzalez, Javier
dc.contributor.authorTroncoso, Jose
dc.contributor.authorKoop, Ben F.
dc.contributor.authorWadsworth, Simon L.
dc.contributor.authorFast, Mark D.
dc.date.accessioned2018-08-27T14:35:06Z
dc.date.available2018-08-27T14:35:06Z
dc.date.copyright2017en_US
dc.date.issued2017
dc.description.abstractSalmon lice (Lepeophtheirus salmonis) are important ectoparasites of wild and farmed salmonids and cause major losses to the salmon farming industry throughout the Northern Hemisphere. With the emergence of resistance to several commonly used parasiticides, novel control strategies and integration of multiple treatment options are needed, including host immunostimulation. Here, we investigate the effects of a functional feed containing a peptidoglycan and nucleotide formulation on L. salmonis infection of Atlantic salmon (Salmo salar) by characterizing lice infection levels, the expression of several host immune genes, and the parasite transcriptomic response to the immunostimulated host. Although initial infection intensities were low, the low dose (LD) immunostimulant diet reduced the total lice burden by 50% relative to controls. Immunostimulant fed hosts upregulated interleukin-1 beta in the skin and spleen. This gene has been implicated in successful responses of several salmonid species to salmon lice but is typically not observed in Atlantic salmon, suggesting a favorable influence on the immune response. Lice infecting LD immunostimulated salmon overexpressed genes putatively involved in parasite immunity, including carboxylesterases, and underexpressed genes putatively involved in feeding (e.g., proteases). These lice response genes further improve the characterization of the transcriptome of the non-model parasite by identifying genes potentially involved in evading host immunity.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe authors would like to thank EWOS Innovation, Novartis Animal Health Canada (Dr. Fast's Research Chair in fish health), NSERC Engage and Discovery programs, and Innovation PEI (student and PDF scholarships) for funding this research; Department of Pathology and Microbiology and Merial scholarships at AVC-UPEI for summer research interns; and the Aquatics Facilities and Staff at the Atlantic Veterinary College for their role in maintaining the Atlantic salmon. BJGS was supported during this work by NSERC-CGS and PDF fellowships.en_US
dc.identifier.citationSutherland, B.J.G.; Covello, J.M.; Friend, S.E.; Poley, J.D.; Koczka, K.W.; Purcell, S.L.; … & Fast, M.D. (2017). Host–parasite transcriptomics during immunostimulantenhanced rejection of salmon lice (Lepeophtheirus salmonis) by Atlantic salmon (Salmo salar). FACETS, 2, 477-495. doi: 10.1139/facets-2017-0020en_US
dc.identifier.urihttps://doi.org/10.1139/facets-2017-0020
dc.identifier.urihttp://hdl.handle.net/1828/9957
dc.language.isoenen_US
dc.publisherFACETSen_US
dc.subjectfunctional feeds
dc.subjecthost-parasite interaction
dc.subjectimmunostimulation
dc.subjectsalmon aquaculture
dc.subjectsea lice
dc.subjecttranscriptomics
dc.subjectCentre for Biomedical Research
dc.subject.departmentDepartment of Biology
dc.titleHost–parasite transcriptomics during immunostimulant-enhanced rejection of salmon lice (Lepeophtheirus salmonis) by Atlantic salmon (Salmo salar)en_US
dc.typeArticleen_US

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