Identification and Validation of Potential New Biomarkers for Prostate Cancer Diagnosis and Prognosis Using 2D-DIGE and MS

Date

2015

Authors

Geisler, Cordelia
Gaisa, Nadine T.
Pfister, David
Fuessel, Susanne
Kristiansen, Glen
Braunschweig, Till
Gostek, Sonja
Beine, Birte
Diehl, Hanna C.
Jackson, Angela M.

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Journal ISSN

Volume Title

Publisher

BioMed Research International

Abstract

This study was designed to identify and validate potential new biomarkers for prostate cancer and to distinguish patients with and without biochemical relapse. Prostate tissue samples analyzed by 2D-DIGE (two-dimensional difference in gel electrophoresis) and mass spectrometry (MS) revealed downregulation of secernin-1 (Ρ < 0.044) in prostate cancer, while vinculin showed significant upregulation (Ρ < 0.001). Secernin-1 overexpression in prostate tissue was validated using Western blot and immunohistochemistry while vinculin expression was validated using immunohistochemistry. These findings indicate that secernin-1 and vinculin are potential new tissue biomarkers for prostate cancer diagnosis and prognosis, respectively. For validation, protein levels in urine were also examined by Western blot analysis. Urinary vinculin levels in prostate cancer patients were significantly higher than in urine from nontumor patients (Ρ = 0.006). Using multiple reaction monitoring-MS (MRM-MS) analysis, prostatic acid phosphatase (PAP) showed significant higher levels in the urine of prostate cancer patients compared to controls (Ρ = 0.012), while galectin-3 showed significant lower levels in the urine of prostate cancer patients with biochemical relapse, compared to those without relapse (Ρ = 0.017). Three proteins were successfully differentiated between patients with and without prostate cancer and patients with and without relapse by using MRM. Thus, this technique shows promise for implementation as a noninvasive clinical diagnostic technique.

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Citation

Geisler, C., Gaisa, N.T., Pfister, D., Fuessel, S., Kristiansen, G., Braunschweig, T., …, & Henkel, C. (2015). Identification and validation of potential new biomarkers for prostate cancer diagnosis and prognosis using 2D-DIGE and MS. BioMed Research International, Vol. 2015, Article ID 454256.