Identification and Validation of Potential New Biomarkers for Prostate Cancer Diagnosis and Prognosis Using 2D-DIGE and MS

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dc.contributor.authorGeisler, Cordelia
dc.contributor.authorGaisa, Nadine T.
dc.contributor.authorPfister, David
dc.contributor.authorFuessel, Susanne
dc.contributor.authorKristiansen, Glen
dc.contributor.authorBraunschweig, Till
dc.contributor.authorGostek, Sonja
dc.contributor.authorBeine, Birte
dc.contributor.authorDiehl, Hanna C.
dc.contributor.authorJackson, Angela M.
dc.contributor.authorBorchers, Christoph H.
dc.contributor.authorHeidenreich, Axel
dc.contributor.authorMeyer, Helmut E.
dc.contributor.authorKnüchel, Ruth
dc.contributor.authorHenkel, Corinna
dc.date.accessioned2017-10-23T17:47:54Z
dc.date.available2017-10-23T17:47:54Z
dc.date.copyright2015en_US
dc.date.issued2015
dc.description.abstractThis study was designed to identify and validate potential new biomarkers for prostate cancer and to distinguish patients with and without biochemical relapse. Prostate tissue samples analyzed by 2D-DIGE (two-dimensional difference in gel electrophoresis) and mass spectrometry (MS) revealed downregulation of secernin-1 (Ρ < 0.044) in prostate cancer, while vinculin showed significant upregulation (Ρ < 0.001). Secernin-1 overexpression in prostate tissue was validated using Western blot and immunohistochemistry while vinculin expression was validated using immunohistochemistry. These findings indicate that secernin-1 and vinculin are potential new tissue biomarkers for prostate cancer diagnosis and prognosis, respectively. For validation, protein levels in urine were also examined by Western blot analysis. Urinary vinculin levels in prostate cancer patients were significantly higher than in urine from nontumor patients (Ρ = 0.006). Using multiple reaction monitoring-MS (MRM-MS) analysis, prostatic acid phosphatase (PAP) showed significant higher levels in the urine of prostate cancer patients compared to controls (Ρ = 0.012), while galectin-3 showed significant lower levels in the urine of prostate cancer patients with biochemical relapse, compared to those without relapse (Ρ = 0.017). Three proteins were successfully differentiated between patients with and without prostate cancer and patients with and without relapse by using MRM. Thus, this technique shows promise for implementation as a noninvasive clinical diagnostic technique.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThe authors thank the FoRUM funding of the Ruhr University Bochum and the START funding of the University Hospital Aachen for financial support. Hanna C. Diehl and Birte Beine and Corinna Henkel were additionally supported by PURE (Protein research Unit Ruhr within Europe). Angela M. Jackson and Christoph H. Borchers are grateful to Genome Canada and Genome BC for support of the University of Victoria-Genome BC Proteomics Centre through funding of the Science and Technology Innovation Centre.en_US
dc.identifier.citationGeisler, C., Gaisa, N.T., Pfister, D., Fuessel, S., Kristiansen, G., Braunschweig, T., …, & Henkel, C. (2015). Identification and validation of potential new biomarkers for prostate cancer diagnosis and prognosis using 2D-DIGE and MS. BioMed Research International, Vol. 2015, Article ID 454256.en_US
dc.identifier.urihttp://dx.doi.org/10.1155/2015/454256
dc.identifier.urihttp://hdl.handle.net/1828/8710
dc.language.isoenen_US
dc.publisherBioMed Research Internationalen_US
dc.titleIdentification and Validation of Potential New Biomarkers for Prostate Cancer Diagnosis and Prognosis Using 2D-DIGE and MSen_US
dc.typeArticleen_US

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