The effect of controlled growth factor delivery on embryonic stem cell differentiation inside fibrin scaffolds

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dc.contributor.authorWillerth, Stephanie
dc.contributor.authorRader, Allison
dc.contributor.authorSakiyama-Elbert, Shelly E
dc.date.accessioned2015-07-03T21:24:13Z
dc.date.available2015-07-03T21:24:13Z
dc.date.copyright2008en_US
dc.date.issued2008-06-10
dc.description.abstractThe goal of this project was to develop 3-D biomaterial scaffolds that present cues to direct the differentiation of embryonic stem (ES) cell-derived neural progenitor cells, seeded inside the scaffolds, into mature neural phenotypes, specifically neurons and oligodendrocytes. Release studies were performed to determine the appropriate conditions for retention of neurotrophin-3 (NT-3), sonic hedgehog, and platelet-derived growth factor (PDGF) by an affinity-based delivery system incorporated into fibrin scaffolds. Embryoid bodies containing neural progenitors were formed from mouse ES cells, using a 4−/4+ retinoic acid treatment protocol, and then seeded inside fibrin scaffolds containing the drug delivery system. This delivery system was used to deliver various growth factor doses and combinations to the cells seeded inside the scaffolds. Controlled delivery of NT-3 and PDGF simultaneously increased the fraction of neural progenitors, neurons, and oligodendrocytes while decreasing the fraction of astrocytes obtained compared to control cultures seeded inside unmodified fibrin scaffolds with no growth factors present in the medium. These results demonstrate that such a strategy can be used to generate an engineered tissue for the potential treatment of spinal cord injury and could be extended to the study of differentiation in other tissues.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by NIH R01 NS051454. The authors thank Amy Boyet, Angela Guzmán, Nicole Moore, and Matthew Wood for technical support. The authors thank Shengzhou Wu and the Hope Center for Neurological Disorders, which is supported by an NIH Neuroscience Blueprint Interdisciplinary Center Core grant (P30 NS057105), for use of the real-time PCR machine.en_US
dc.identifier.citationWillerth, SM et al. The effect of controlled growth factor delivery on embryonic stem cell differentiation inside fibrin scaffolds Stem Cell Res 1(3): 205-218en_US
dc.identifier.urihttp://www.sciencedirect.com/science/article/pii/S187350610800038X
dc.identifier.urihttp://dx.doi.org/10.1016/j.scr.2008.05.006
dc.identifier.urihttp://hdl.handle.net/1828/6287
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectmurine embryonic stem cell
dc.subject3D culture
dc.subjecthydrogel
dc.subjectneural tissue engineering
dc.subject.departmentDepartment of Mechanical Engineering
dc.subject.departmentSchool of Medical Sciences
dc.titleThe effect of controlled growth factor delivery on embryonic stem cell differentiation inside fibrin scaffoldsen_US
dc.typeArticleen_US

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