Biomarkers and gene copy number variation for terpenoid traits associated with insect resistance in Sitka spruce : an integrated genomic, proteomic, and biochemical anaysis of (+)-3-carene biosynthesis

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dc.contributor.authorBohlmann, Joerg
dc.contributor.authorHall, Dawn
dc.contributor.authorRobert, Jeanne
dc.contributor.authorKeeling, Christopher
dc.contributor.authorDomanski, Dominik
dc.contributor.authorQuesada, Alfonso Lara
dc.contributor.authorJancsik, Sharon
dc.contributor.authorHamberger, Britta
dc.contributor.authorBorchers, Christoph
dc.contributor.authorKuzyk, Michael
dc.date.accessioned2013-10-29T20:53:57Z
dc.date.available2013-10-29T20:53:57Z
dc.date.copyright2011en_US
dc.date.issued2011-09-13
dc.descriptionBioMed Centralen_US
dc.description.abstractConifers have evolved complex chemical defenses in the form of oleoresin terpenoids to resist attack from pathogens and herbivores. The large diversity of terpenoid metabolites is determined by the size and composition of the terpene synthase (TPS) gene family, and the single- and multi-product profiles of these enzymes. The monoterpene (+)-3-carene is associated with resistance of Sitka spruce (Picea sitchensis) to white pine weevil (Pissodes strobi). We used a combined genomic, proteomic and biochemical approach to analyze the (+)-3-carene phenotype in two contrasting Sitka spruce genotypes. Resistant trees produced significantly higher levels of (+)-3-carene than susceptible trees, in which only trace amounts were detected. Biosynthesis of (+)-3- carene is controlled, at the genome level, by a small family of closely related (82-95% amino acid sequence identity) (+)-3-carene synthase (PsTPS-3car) genes. Transcript profiling identified one PsTPS-3car gene (PsTPS-3car1) which is expressed in both genotypes, one gene (PsTPS-3car2) expressed only in resistant trees, and one gene (PsTPS-3car3) expressed only in susceptible trees. The PsTPS-3car2 gene was not detected in genomic DNA of susceptible trees. Targetspecific selected reaction monitoring substantiated this pattern of differential expression of members of the PsTPS-3car family on the proteome level. Kinetic characterization of the recombinant PsTPS-3car enzymes identified differences in the activities of PsTPS- 3car2 and PsTPS-3car3as a factor for the different (+)-3-carene profiles of resistant and susceptible trees. In conclusion, variation of the (+)-3-carene phenotype is controlled by PsTPS-3car gene copy number variation, variation of gene and protein expression, and variation of catalytic efficiencies.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.identifier.citationBohlmann et al.: Biomarkers and gene copy number variation for terpenoid traits associated with insect resistance in Sitka spruce: An integrated genomic, proteomic, and biochemical analysis of (+)-3-carene biosynthesis. BMC Proceedings 2011 5(Suppl 7):O27.en_US
dc.identifier.urihttp://www.biomedcentral.com/1753-6561/5/S7/O27
dc.identifier.urihttp://dx.doi.org/10.1186/1753-6561-5-S7-O27
dc.identifier.urihttp://hdl.handle.net/1828/5009
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.subjectUVic Genome BC Proteomics Centre
dc.subject.departmentDepartment of Biochemistry and Microbiology
dc.titleBiomarkers and gene copy number variation for terpenoid traits associated with insect resistance in Sitka spruce : an integrated genomic, proteomic, and biochemical anaysis of (+)-3-carene biosynthesisen_US
dc.typeArticleen_US

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