Comprehensive analysis of MHC class II genes in teleost fish genomes reveals dispensability of the peptide-loading DM system in a large part of vertebrates

Simple item page
dc.contributor.authorDijkstra, Johannes M
dc.contributor.authorGrimholt, Unni
dc.contributor.authorLeong, Jong
dc.contributor.authorKoop, Ben F
dc.contributor.authorHashimoto, Keiichiro
dc.date.accessioned2015-03-04T22:00:28Z
dc.date.available2015-03-04T22:00:28Z
dc.date.copyright2013en_US
dc.date.issued2013-11-26
dc.descriptionBioMed Centralen_US
dc.description.abstractBackground: Classical major histocompatibility complex (MHC) class II molecules play an essential role in presenting peptide antigens to CD4+ T lymphocytes in the acquired immune system. The non-classical class II DM molecule, HLA-DM in the case of humans, possesses critical function in assisting the classical MHC class II molecules for proper peptide loading and is highly conserved in tetrapod species. Although the absence of DM-like genes in teleost fish has been speculated based on the results of homology searches, it has not been definitively clear whether the DM system is truly specific for tetrapods or not. To obtain a clear answer, we comprehensively searched class II genes in representative teleost fish genomes and analyzed those genes regarding the critical functional features required for the DM system. Results: We discovered a novel ancient class II group (DE) in teleost fish and classified teleost fish class II genes into three major groups (DA, DB and DE). Based on several criteria, we investigated the classical/non-classical nature of various class II genes and showed that only one of three groups (DA) exhibits classical-type characteristics. Analyses of predicted class II molecules revealed that the critical tryptophan residue required for a classical class II molecule in the DM system could be found only in some non-classical but not in classical-type class II molecules of teleost fish. Conclusions: Teleost fish, a major group of vertebrates, do not possess the DM system for the classical class II peptide-loading and this sophisticated system has specially evolved in the tetrapod lineage.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by JSPS KAKENHI Grant Number 22580213 and 25450310 (to J. M. D.), a Norwegian Research Council grant (Havbruk # NFR 185217/S40) (to U. G.), an NSERC grant (to B. F. K.) and the MEXT-Supported Program for the Strategic Research Foundation at Private Universities, 2008–2012.en_US
dc.identifier.citationDijkstra et al.: Comprehensive analysis of MHC class II genes in teleost fish genomes reveals dispensability of the peptideloading DM system in a large part of vertebrates. BMC Evolutionary Biology 2013 13:260.en_US
dc.identifier.urihttp://www.biomedcentral.com/1471-2148/13/260
dc.identifier.urihttp://dx.doi.org/10.1186/1471-2148-13-260
dc.identifier.urihttp://hdl.handle.net/1828/5895
dc.language.isoenen_US
dc.publisherBMC Evolutionary Biologyen_US
dc.rights.tempAttribution-NonCommercial-NoDerivs 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ca/*
dc.subjectMHC
dc.subjectClass II
dc.subjectClassical
dc.subjectNon-classical
dc.subjectDM
dc.subjectPeptide-loading
dc.subjectTeleost fish
dc.subjectGenomics
dc.subjectEvolution
dc.subjectCentre for Biomedical Research
dc.subject.departmentDepartment of Biology
dc.titleComprehensive analysis of MHC class II genes in teleost fish genomes reveals dispensability of the peptide-loading DM system in a large part of vertebratesen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Dijkstra_Johannes_BMCEvolBiol_2013.pdf
Size:
1.71 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.74 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Faculty and Staff Publications
 
University of Victoria Libraries

We acknowledge and respect the Lək̓ʷəŋən (Songhees and Esquimalt) Peoples on whose territory the university stands, and the Lək̓ʷəŋən and W̱SÁNEĆ Peoples whose historical relationships with the land continue to this day.