Evolution of a Secondary Metabolic Pathway from Primary Metabolism: Shikimate and quinate biosynthesis in plants

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dc.contributor.authorCarrington, Yuriko
dc.contributor.authorGuo, Jia
dc.contributor.authorLe, Cuong H.
dc.contributor.authorFillo, Alexander
dc.contributor.authorKwon, Junsu
dc.contributor.authorTran, Lan T.
dc.contributor.authorEhlting, Jurgen
dc.date.accessioned2018-11-02T08:09:06Z
dc.date.copyright2018en_US
dc.date.issued2018-06
dc.description.abstractThe shikimate pathway synthesizes aromatic amino acids essential for protein biosynthesis. Shikimate dehydrogenase (SDH) is a central enzyme of this primary metabolic pathway, producing shikimate. The structurally similar quinate is a secondary metabolite synthesized by quinate dehydrogenase (QDH). SDH and QDH belong to the same gene family, which diverged into two phylogenetic clades after a defining gene duplication just prior to the angiosperm/gymnosperm split. Non-seed plants that diverged before this duplication harbour only a single gene of this family. Extant representatives from the chlorophytes (Chlamydomonas reinhardtii), bryophytes (Physcomitrella patens) and lycophytes (Selaginella moellendorfii) encoded almost exclusively SDH activity in vitro. A reconstructed ancestral sequence representing the node just prior to the gene duplication also encoded SDH activity. Quinate dehydrogenase activity was gained only in seed plants following gene duplication. Quinate dehydrogenases of gymnosperms, represented here by Pinus taeda, may be reminiscent of an evolutionary intermediate since they encode equal SDH and QDH activities. The second copy in P. taeda maintained specificity for shikimate similar to the activity found in the angiosperm SDH sister clade. The codon for a tyrosine residue within the active site displayed a signature of positive selection at the node defining the QDH clade, where it changed to a glycine. Replacing the tyrosine with a glycine in a highly shikimate-specific angiosperm SDH was sufficient to gain some QDH function. Thus, very few mutations were necessary to facilitate the evolution of QDH genes.en_US
dc.description.embargo2019-07-01
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work has been supported by a Discovery Grant (to JE) from the Natural Sciences and Engineering Research Council of Canada (NSERC). YC was supported by a stipend from the NSERC Collaborative Research and Training Experience (CREATE) Program in Forests and Climate Change. AF and JK received undergraduate research project awards from the Centre for Forest Biology at the University of Victoria. We appreciate early access to the 1KP transcriptome database.en_US
dc.identifier.citationCarrington, Y., Guo, J., Le, C.H., Fillo, A., Kwon, J., Tran, L.T. & Ehlting, J. (2018). Evolution of a secondary metabolic pathway from primary metabolism: shikimate and quinate biosynthesis in plants. The Plant Journal, 95(5), 823-833. https://doi.org/10.1111/tpj.13990en_US
dc.identifier.urihttps://doi.org/10.1111/tpj.13990
dc.identifier.urihttp://hdl.handle.net/1828/10229
dc.language.isoenen_US
dc.publisherthe Plant Journalen_US
dc.subjectmolecular evolutionen_US
dc.subjectsecondary metabolismen_US
dc.subjectshikimate/quinate dehydrogenaseen_US
dc.subjectRhodopirellula balticaen_US
dc.subjectChlamydomonas reinhardtiien_US
dc.subjectPhyscomitrella patensen_US
dc.subjectSelaginella moellendorfiien_US
dc.subjectPinus taedaen_US
dc.subjectPopulus trichocarpaen_US
dc.titleEvolution of a Secondary Metabolic Pathway from Primary Metabolism: Shikimate and quinate biosynthesis in plantsen_US
dc.typePostprinten_US

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