Complete nucleotide sequence of the cosmid vector pWE15A
| dc.contributor.author | Seto, Donald | |
| dc.contributor.author | Koop, Ben F. | |
| dc.contributor.author | Seto, Jason | |
| dc.contributor.author | Hood, Larry | |
| dc.date.accessioned | 2015-08-25T20:12:00Z | |
| dc.date.available | 2015-08-25T20:12:00Z | |
| dc.date.copyright | 1992 | en_US |
| dc.date.issued | 1992 | |
| dc.description.abstract | Current efforts to determine the complete nucleotide sequence of the six murine and human T-cell receptor (TCR) loci are limited by the availability of overlapping genomic inserts cloned into a defined and characterized vector. In order to create these mapping and sequencing libraries, our laboratory has modified an existing cosmid vector pWE15 (1,2). The optimized version, pWE15A (3), contains a polylinker with 15 infrequently cleaved restriction enzyme sites, asymmetrically centered around the BamHI site, which is the cloning site used for the insertion of the genomic DNA described below. The modifications allow recovery of inserted DNA by the use of several restriction enzymes. Each recombinant insert bearing vector may contain sequenceable genomic DNA ranging in size from 35 to 40 kilobases (kb). This complete 8213 nucleotide pWE15A sequence complements the two noncontiguous approximately 2 kb of pWE15A extant in GenBank. | |
| dc.description.reviewstatus | Reviewed | en_US |
| dc.description.scholarlevel | Faculty | en_US |
| dc.description.sponsorship | GM: 13039; DOE: FG0391ER61182; NIH: HG00356; | en_US |
| dc.identifier.citation | Seto, Donald et al. (1992) Complete nucleotide sequence of the cosmid vector pWE15A. Nucleic Acid Research. 20 (14), p. 3786. | en_US |
| dc.identifier.uri | http://dx.doi.org/10.1093/nar/20.14.3786 | |
| dc.identifier.uri | http://hdl.handle.net/1828/6542 | |
| dc.language.iso | en | en_US |
| dc.subject.department | Department of Biology | |
| dc.title | Complete nucleotide sequence of the cosmid vector pWE15A | en_US |
| dc.type | Article | en_US |