Microglia/macrophages are ultrastructurally altered by their proximity to spinal cord injury in adult female mice

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dc.contributor.authorSt-Pierre, Marie-Kim
dc.contributor.authorGonzález Ibáñez, Fernando
dc.contributor.authorKroner, Antje
dc.contributor.authorTremblay, Marie-Ève
dc.date.accessioned2024-01-08T22:29:40Z
dc.date.available2024-01-08T22:29:40Z
dc.date.copyright2023en_US
dc.date.issued2023
dc.description.abstractTraumatic spinal cord injury can cause immediate physical damage to the spinal cord and result in severe neurological deficits. The primary, mechanical tissue damage triggers a variety of secondary damage mechanisms at the injury site which significantly contribute to a larger lesion size and increased functional damage. Inflammatory mechanisms which directly involve both microglia (MG) and monocyte-derived macrophages (MDM) play important roles in the post-injury processes, including inflammation and debris clearing. In the current study, we investigated changes in the structure and function of MG/MDM in the injured spinal cord of adult female mice, 7 days after a thoracic contusion SCI. With the use of chip mapping scanning electron microscopy, which allows to image large samples at the nanoscale, we performed an ultrastructural comparison of MG/MDM located near the lesion vs adjacent regions to provide novel insights into the mechanisms at play post-injury. We found that MG/MDM located near the lesion had more mitochondria overall, including mitochondria with and without morphological alterations, and had a higher proportion of altered mitochondria. MG/MDM near the lesion also showed an increased number of phagosomes, including phagosomes containing myelin and partiallydigested materials. MG/MDM near the injury interacted differently with the spinal cord parenchyma, as shown by their reduced number of direct contacts with synaptic elements, axon terminals and dendritic spines. In this study, we characterized the ultrastructural changes of MG/MDM in response to spinal cord tissue damage in mice, uncovering changes in phagocytic activity, mitochondrial ultrastructure, and inter-cellular interactions within the spinal cord parenchyma.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipMKSP was supported by doctoral training awards from the Canadian Institutes of Health Research (CIHR) and Fonds de recherche du Québec-Santé (FRQS). FGI was supported by a full doctoral scholarship from the Mexican Council of Science and Technology (CONACYT). AK received funding from Advancing a Healthier Wisconsin (AHW). MET holds a Canada Research Chair (Tier 2) in Neurobiology of Aging and Cognition. This research was also funded by a Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery grant awarded to MET (RGPIN-2014-05308), the Canada Foundation for Innovation John R. Evans Leaders Fund (Grant 39965, Laboratory of ultrastructural insights into the neurobiology of aging and cognition) and Canada Research Chair funding (CRC-2019-00407).en_US
dc.identifier.citationSt-Pierre, M-K., González Ibáñez, F., Kroner, A., & Tremblay, M-È. (2023). Microglia/macrophages are ultrastructurally altered by their proximity to spinal cord injury in adult female mice. Journal of Neuroinflammation, 20, 273. https://doi.org/10.1186/s12974-023-02953-0en_US
dc.identifier.urihttps://doi.org/10.1186/s12974-023-02953-0
dc.identifier.urihttp://hdl.handle.net/1828/15810
dc.language.isoenen_US
dc.publisherJournal of Neuroinflammationen_US
dc.subjectSpinal cord injury
dc.subjectUltrastructure
dc.subjectMicroglia
dc.subjectPeripheral macrophages
dc.subjectPhagocytosis
dc.subjectCellular stress
dc.subjectMetabolism
dc.subjectMyelinated axons
dc.subjectSynaptic interactions
dc.subjectMouse
dc.subjectCentre for Advanced Materials and Related Technology (CAMTEC)
dc.subjectInstitute on Aging and Lifelong Health
dc.subject.departmentDivision of Medical Sciences
dc.subject.departmentSchool of Medical Sciences
dc.titleMicroglia/macrophages are ultrastructurally altered by their proximity to spinal cord injury in adult female miceen_US
dc.typeArticleen_US

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