DNA Sequence Homology between the Terminal Inverted Repeats of Shope Fibroma Virus and an Endogenous Cellular Plasmid Species

dc.contributor.authorUpton, Chris
dc.contributor.authorMcFadden, Grant
dc.date.accessioned2015-07-06T18:16:08Z
dc.date.available2015-07-06T18:16:08Z
dc.date.copyright1986en_US
dc.date.issued1986
dc.description.abstractDNA hybridization experiments indicate that the genome of a tumorigenic poxvirus. Shope fibroma virus (SFV), possesses sequence homology with DNA isolated from uninfected rabbit cells. Southern blotting experiments, either with high-complexity rabbit DNA as probe and SFV restriction fragments as targets or with high-specific activity, 32P-labeled, cloned SFV sequences as probes and rabbit DNA as target, indicate that the homologous sequences map at two locations within the viral genome, one in each copy of the terminal inverted repeat sequences. Unexpectedly, Southern blots revealed that the homologous host sequences reside in a rabbit extrachromosomal DNA element. This autonomous low-molecular-weight DNA species could be specifically amplified by cycloheximide treatment and was shown by isopycnic centrifugation in cesium chloride-ethidium bromide to consist predominantly of covalently closed circular DNA molecules. DNA sequencing of pSIC-9, a cloned 1.9-kilobase fragment of the rabbit plasmid species, indicated extensive homology at the nucleotide level over a 1.5-kilobase stretch of the viral terminal inverted repeat. Analysis of open reading frames in both the plasmid and SFV DNA revealed that (i) the N-terminal 157-amino acid sequence of a potential 514-amino acid SFV polypeptide is identical to the N-terminal 157 amino acids of one pSIC-9 open reading frame, and (ii) a second long pSIC-9 open reading frame of 361 amino acids, although significantly diverged from the comparable nucleotide sequence in the virus, possessed considerable homology to a family of cellular protease inhibitors, including alpha 1-antichymotrypsin, alpha 1-antitrypsin, and antithrombin III. The potential role of such cellular plasmid-like DNA species as a mediator in the exchange of genetic information between the host cell and a cytoplasmically replicating poxvirus is discussed.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was supported by the Alberta Heritage Foundation for Medical Research (AHFMR) and the National Cancer Institute of Canada. Computer costs of the BIONET resource were funded by Public Health Service grant 1-441-RR01685-01 from the National Institutes of Health. G.M. is an AHFMR scholar, and C.U. is an AHFMR postdoctoral fellow.en_US
dc.identifier.citationC Upton and G McFadden. Mol. Cell. Biol. January 1986 vol. 6 no. 1 p.265-276en_US
dc.identifier.urihttp://mcb.asm.org/content/6/1/265.abstract
dc.identifier.urihttp://hdl.handle.net/1828/6294
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.titleDNA Sequence Homology between the Terminal Inverted Repeats of Shope Fibroma Virus and an Endogenous Cellular Plasmid Speciesen_US
dc.typeArticleen_US

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