Identification of novel miRNAs and miRNA expression profiling in embryogenic tissues of Picea balfouriana treated by 6-benzylaminopurine

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dc.contributor.authorLi, Qingfen
dc.contributor.authorDeng, Cheng
dc.contributor.authorXia, Yan
dc.contributor.authorKong, Lisheng
dc.contributor.authorZhang, Hanguo
dc.contributor.authorZhang, Shougong
dc.contributor.authorWang, Junhui
dc.date.accessioned2018-08-20T19:15:50Z
dc.date.available2018-08-20T19:15:50Z
dc.date.copyright2017en_US
dc.date.issued2017
dc.description.abstractHere, we compared miRNA expression profiles in embryonic cell cultures of the conifer Picea balfouriana following application of the synthetic cytokinin 6-benzylaminopurine (6-BAP). We used next-generation sequencing to analyze three libraries of small RNAs from the treated embryogenic cell cultures and generated 24,000,000 raw reads from each of the libraries. Over 70 differentially regulated micro RNA (miRNA) families (>= 2 fold change in expression) were identified between pairs of treatments. A quantitative analysis showed that miR3633 and miR1026 were upregulated in tissues with the highest embryogenic ability. These two miRNAs were predicted to target genes encoding receptor-like protein kinase and GAMYB transcription factors, respectively. In one library, miR1160, miR5638, miR1315, and miR5225 were downregulated. These four miRNAs were predicted to target genes encoding APETALA2, calmodulin-binding protein, and calciumdependent protein kinase transcription factors. The expression patterns of the miRNAs and their targets were negatively correlated. Approximately 181 potentially novel P. balfouriana miRNAs were predicted from the three libraries, and seven were validated during the quantitative analysis. This study is the first report of differential miRNA regulation in tissues treated with 6-BAP during somatic embryogenesis. The differentially expressed miRNAs will be of value for investigating the mechanisms of embryogenic processes that are responsive to 6-BAP in P. balfouriana.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipFinancial support for this study was provided by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF, China (TGB2013011).en_US
dc.identifier.citationLi, Q.; Deng, C.; Xia, Y.; Kong, L.; Zhang, H.; Zhang, S.; & Wang, J. (2017). Identification of novel miRNAs and miRNA expression profiling in embryogenic tissues of Picea balfouriana treated by 6-benzylaminopurine. PLoS One, 12(5), e0176112. https://doi.org/10.1371/journal.pone.0176112en_US
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0176112
dc.identifier.urihttp://hdl.handle.net/1828/9935
dc.language.isoenen_US
dc.publisherPLoS Oneen_US
dc.subjectCentre for Forest Biology
dc.subject.departmentDepartment of Biology
dc.titleIdentification of novel miRNAs and miRNA expression profiling in embryogenic tissues of Picea balfouriana treated by 6-benzylaminopurineen_US
dc.typeArticleen_US

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