3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink

dc.contributor.authorRestan Perez, Milena
dc.contributor.authorSharma, Ruchi
dc.contributor.authorZeina Masri, Nadia
dc.contributor.authorWillerth, Stephanie
dc.date.accessioned2021-10-01T22:17:45Z
dc.date.available2021-10-01T22:17:45Z
dc.date.copyright2021en_US
dc.date.issued2021
dc.description.abstractCurrent treatments for neurodegenerative diseases aim to alleviate the symptoms experienced by patients; however, these treatments do not cure the disease nor prevent further degeneration. Improvements in current disease-modeling and drug-development practices could accelerate effective treatments for neurological diseases. To that end, 3D bioprinting has gained significant attention for engineering tissues in a rapid and reproducible fashion. Additionally, using patient-derived stem cells, which can be reprogrammed to neural-like cells, could generate personalized neural tissues. Here, adipose tissue-derived mesenchymal stem cells (MSCs) were bioprinted using a fibrin-based bioink and the microfluidic RX1 bioprinter. These tissues were cultured for 12 days in the presence of SB431542 (SB), LDN-193189 (LDN), purmorphamine (puro), fibroblast growth factor 8 (FGF8), fibroblast growth factor-basic (bFGF), and brain-derived neurotrophic factor (BDNF) to induce differentiation to dopaminergic neurons (DN). The constructs were analyzed for expression of neural markers, dopamine release, and electrophysiological activity. The cells expressed DN-specific and early neuronal markers (tyrosine hydroxylase (TH) and class III beta-tubulin (TUJ1), respectively) after 12 days of differentiation. Additionally, the tissues exhibited immature electrical signaling after treatment with potassium chloride (KCl). Overall, this work shows the potential of bioprinting engineered neural tissues from patient-derived MSCs, which could serve as an important tool for personalized disease models and drug-screening.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was funded by the NSERC Discovery Grant program, NSERC Idea to Innovation program, Innovate BC’s Ignite program, the Alzheimer’s Association, Canada Research Chairs and the Michael Smith Foundation for Health Research and Pacific Parkinson’s Research Institute’s Innovation to Commercialization grant.en_US
dc.identifier.citation3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink. (2021). 3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink. Biomolecules, 11(8), 1-15. https://doi.org/10.3390/biom11081250.en_US
dc.identifier.urihttps://doi.org/10.3390/biom11081250
dc.identifier.urihttp://hdl.handle.net/1828/13434
dc.language.isoenen_US
dc.publisherBiomoleculesen_US
dc.subject3D bioprintingen_US
dc.subjectfibrinen_US
dc.subjectsmall moleculesen_US
dc.subjectneural tissuesen_US
dc.subjectstem cellsen_US
dc.title3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioinken_US
dc.typeArticleen_US

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