Transcriptome Analysis Provides Insight into Venom Evolution in a Seed-Parasitic Wasp, Megastigmus spermotrophus

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dc.contributor.authorPaulson, Amber
dc.contributor.authorLe, Cuong
dc.contributor.authorDickson, Jamie
dc.contributor.authorEhlting, Jürgen
dc.contributor.authorvon Aderkas, Patrick
dc.contributor.authorPerlman, Steve
dc.date.accessioned2017-07-31T17:44:49Z
dc.date.available2017-07-31T17:44:49Z
dc.date.copyright2016en_US
dc.date.issued2016-10
dc.description.abstractOne of the most striking host range transitions is the evolution of plant parasitism from animal parasitism. Parasitoid wasps that have secondarily evolved to attack plants (ie gall wasps and seed-feeders) demonstrate intimate associations with their hosts, yet the mechanism of plant–host manipulation is currently not known. There is, however, emerging evidence suggesting that ovipositional secretions play a role in plant manipulation. To investigate whether parasites have modified pre-existing adaptations to facilitate dramatic host shifts we aimed to characterize the expression of venom proteins in a plant parasite using a collection of parasitoid venom sequences as a guide. The transcriptome of a seed-feeding wasp, Megastigmus spermotrophus, was assembled de novo and three putative venoms were found to be highly expressed in adult females. One of these putative venoms, aspartylglucosaminidase, has been previously identified as a major venom component in two distantly related parasitoid wasps (Asobara tabida and Leptopilina heterotoma) and may have originated via gene duplication within the Hymenoptera. Our study shows that M. spermotrophus, a specialized plant parasite, expresses putative venom transcripts that share homology to venoms identified in Nasonia vitripennis (both superfamily Chalcidoidea), which suggests that M. spermotrophus may have co-opted pre-existing machinery to develop as a plant parasite.en_US
dc.description.reviewstatusRevieweden_US
dc.description.scholarlevelFacultyen_US
dc.description.sponsorshipThis work was funded by the Strategic Project Partnership Grants Program of NSERC and the Agence Nationale de Recherche - Programme Blanc 456 International.en_US
dc.identifier.citationPaulson, A.R., Le, C.H., Dickson, J.C., Ehlting, J., von Aderkas, P. & Perlman, S. J. (2016). Transcriptome analysis provides insight into venom evolution in a seedparasitic wasp, Megastigmus spermotrophus. Insect Molecular Biology, 25(5), 604- 616. https://doi.org/10.1111/imb.12247en_US
dc.identifier.urihttps://doi.org/10.1111/imb.12247
dc.identifier.urihttp://hdl.handle.net/1828/8388
dc.language.isoenen_US
dc.publisherInsect Molecular Biologyen_US
dc.subjectvenomen_US
dc.subjectphytophagyen_US
dc.subjectseed parasitismen_US
dc.subjectChalcidoideaen_US
dc.subjectparasitoiden_US
dc.subjectHymenopteraen_US
dc.subjectaspartylglucosaminidaseen_US
dc.titleTranscriptome Analysis Provides Insight into Venom Evolution in a Seed-Parasitic Wasp, Megastigmus spermotrophusen_US
dc.typePreprinten_US

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